By ALFRED GELLHORN, M.D., AND LOGAN 0. JONES, M.D. E VIDENCE from animal experimentation indicates that pyridoxine, a cornponent of the vitamin B complex, is an essential factor for the maintenance and function of hematopoietic tissue. Severe anemia has been induced in dogs’ and swine2 on a pyridoxine deficient diet and the integrity of lymphoid tissue in rats has also been demonstrated to be d...

Bovine lymphosarcoma tissue has been extracted with low- and high-salt buffers [0.05 M Tris-C1 plus or minus 0.3 M (NH4) 2S04]. Diethylaminoethyl-Sephadex chromatography of both the high-salt and low-salt extracts yields RNA polymerases I and II, although low-salt extraction releases only one-third as much activity. Extraction by high salt of the residue from the low-salt extract, followed by d...

Rabbit antisera to rat lymphosarcoma contain antibodies that are cytolytic for rat erythrocytes in the presence of complement. The reaction can be inhibited completely by pure cytolipin R showing that (u) immune hemolysis can be mediated through lipid determinants in the membrane, and ( b ) that cytolipin R determinants are present in the intact erythrocyte membrane and exposed on the surface. ...