Superoxide and other oxygen radicals produced by activated polymorphonuclear leukocytes (PMN) may be important causes of tissue damage in a number ofinflammatory conditions. Therefore, a drug which suppresses PMN responses in vivo is potentially important. In vitro, pentoxifylline (PTOX) inhibits superoxide anion production when PMN are stimulated with an activated complement component (C5a Des...

D-Penicillamine inhibited oxidant secretion from human neutrophils after activation by the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMet-Leu-Phe), as assessed by luminol dependent chemiluminescence. In contrast, this drug had little effect on either intracellular oxidant production or lucigenin dependent chemiluminescence activated by the same agonist. The drug was shown to ...

Mammalian spermatozoa generate a variety of reactive oxygen species (ROS), which are thought to play a role during sperm capacitation, acrosome reaction, and oocyte fusion. However, oxidative stress (OS) occurs if the generation of ROS overwhelms the body’s limited antioxidant defenses. Infertility is the main complication associated with high levels of OS in the seminal fluid. Patients with id...

Objective: Epileptic seizures may cause skeletal muscle injury and memory dysfunctions. The present study was aimed to investigate the possible protective effects of exercising prior seizure on seizure-induced oxidative in brain. Materials Methods: Sprague-Dawley male rats were assigned as non-exercise (n=16) exercise groups (n=16). Following a 3-day training, protocol (30 min) performed treadm...

Psoriasis is an immune-mediated inflammatory skin disease mainly driven by interleukin 17A (IL-17A) and associated with cardiovascular dysfunction. Here we used a severe psoriasis mouse model of dermal IL-17A overexpression (K14-IL-17Aind/+, IL-17Aind/+ control mice) to investigate the activity neutrophils potential cellular interconnection between vasculature. Levels reactive oxygen species (R...

Co-oxidation of chlorogenic acid, caffeic acid, aesculetin and lucigenin with linoleic acid and egg phosphatidyl choline leads to the formation of fluorescent polymer materials. The fluorescent products are more lipophylic, they have lower elution volumes on Sephadex LH-20 column than related phenols and they differ by their fluorescence and chromatographic properties considerably from polymer ...

Organochlorine pesticide endosulfan has been detected for the first time by using surface-enhanced Raman scattering (SERS) at trace concentrations. The bis-acridinium dication lucigenine was successfully used as a molecular assembler in the functionalization of metal nanoparticles to facilitate the approach of the pesticide to the metal surface. From the SERS spectra valuable information about ...

The effects of pneumolysin on the proinflammatory activity of human neutrophils, as well as on cation fluxes in these cells, have been investigated. Superoxide production, release of elastase, CR3 expression, phospholipase A2 activity, and alterations in membrane potential were measured by use of lucigenin-enhanced chemiluminescence and colorimetric, flow cytometric, radiometric, and spectroflu...