The structural and functional maturation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is essential for application to pharmaceutical testing, disease modeling, ultimately therapeutic use. Multicellular 3D-tissue platforms have improved hiPSC-CMs, but probing cardiac contractile properties remains challenging in a 3D environment, especially at depth live tissues. Usi...

background: methyl 2-benzimidazole carbamate (carbendazim) is a synthetic fungicide used against organisms that cause different types of plant diseases. it is widely used as a preservative in leather, paint, textile, fruit, and paper industry. it is also used as an anticancer drug in clinical medicine. methods: to evaluate the low dose toxicity of this compound in mammalian tissues, carbendazim...

Nowadays, the consumption of meat and meat products has been increased with modern manufacturing techniques. Due to the economic value, the likelihood of using unauthorized tissue is possible in meat products. The aim of this study was to apply morphological methods for detection of unauthorized tissues in meat sausage. In this study, a total number of 20 samples of different types of sausages ...

introduction and objective: liver is an important body organ without which life is impossible. cyclophosphamide is an alkylating agent widely used as an anti-cancer (“antineoplastic” or “cytotoxic”) chemotherapy drug. the present study aimed to find a suitable antioxidant and examine the effect of vitamin e and selenium on reducing the side effects of cyclophosphamide on liver as a vital organ....

In this study, we use combined two-photon excitation fluorescence (TPEF), second-harmonic generation (SHG) and third-harmonic generation (THG) measurements to image cellular structures of the nematode Caenorhabditis elegans, in vivo. To our knowledge, this is the first time that a THG modality is employed to image live C. elegans specimens. Femtosecond laser pulses (1028 nm) were utilized for e...

Trafficking and regulation of mobile zinc pools influence cellular functions and pathological conditions in multiple organs, including brain, pancreas, and prostate. The quest for a dynamic description of zinc distribution and mobilization in live cells fuels the development of increasingly sophisticated probes. Detection systems that respond to zinc binding with changes of their fluorescence e...

Live recombinant bacteria represent an attractive means to induce both mucosal and systemic immune responses against heterologous antigens. Several models have now been developed and shown to be highly efficient following intranasal immunization. In this review, we describe the two main classes of live recombinant bacteria: generally recognized as safe bacteria and attenuated strains derived fr...

We report a two-photon probe (FS1) which shows a 21-fold two-photon excited fluorescence enhancement in response to H(2)S and can selectively detect H(2)S in a rat hippocampal slice at a depth of 90-190 μm by using two-photon microscopy.