Mass spectrometry imaging has been used here to suggest that changes in membrane structure drive lipid domain formation in mating single-cell organisms. Chemical studies of lipid bilayers in both living and model systems have revealed that chemical composition is coupled to localized membrane structure. However, it is not clear if the lipids that compose the membrane actively modify membrane st...

Membrane contact sites (MCS) are regions of close apposition between membrane-bound organelles. Proteins that occupy MCS display various domain organisation. Among them, lipid transfer proteins (LTPs) frequently contain both structured domains as well intrinsic disorder. In this review, we discuss the roles intrinsically disordered protein (IDPRs) in LTPs other associated with organelle sites. ...

Biomembrane is a place for signal transduction, where protein-membrane interactions are controlled by the membrane environment. This environment could be modified by lipid dynamics, such as interbilayer transport and transbilayer movement, which are governed by lipid transfer proteins and translocase enzymes, respectively. Thus, static and dynamic structural evaluations of the membranes are imp...

The OPM (Orientation of Proteins in Membranes) database currently holds, classifies, and annotates around 14,000 three-dimensional structures transmembrane peripheral membrane proteins peptides positioned with respect to boundaries. Developing uniform templates biological membranes realistic native-like lipid composition would provide a convenient accurate platform for researchers who simulate ...

Lipid rafts are membrane microdomains that function as platforms for signal transduction and membrane trafficking. Tyrosine kinase interacting protein (Tip) of T lymphotropic Herpesvirus saimiri (HVS) is targeted to lipid rafts in T cells and downregulates TCR and CD4 surface expression. Here, we report that the membrane-proximal amphipathic helix preceding Tip's transmembrane (TM) domain media...

Transport proteins are essential for cells in allowing the exchange of substances between and their environment across lipid bilayer forming a tight barrier. Membrane lipids modulate function transmembrane such as transporters two ways: Lipids tightly specifically bound to transport addition they from bulk proteins. This overview summarizes currently available information at ultrastructural lev...

In this review we describe the state-of-the-art of computer simulation studies of lipid membranes. We focus on collective lipid-lipid and lipid-protein interactions that trigger deformations of the natural lamellar membrane state, showing that many important biological processes including self-aggregation of membrane components into domains, the formation of non-lamellar phases, and membrane po...

New methods toward manipulation and study of membrane species within a lipid bilayer platform are desired because lipid bilayers are able to maintain native conformation and function of intrinsic membrane species. This report demonstrates design and use of a bilayer patterned device for separation, sorting, and categorization of membrane biomolecules based on their affinity for different co-exi...

Membrane lipid microdomains (lipid rafts) play an important role in T cell function by forming areas of high lipid order that facilitate activation. However, their role in regulating T cell differentiation and function remains controversial. In this study, by applying a new approach involving microscopy and flow cytometry, we characterize membrane lipid order in ex vivo primary human CD4(+) T c...

In eukaryotic cells, the membranes of different intracellular organelles have different lipid composition, and various biomembranes show an asymmetric distribution of lipid types across the membrane bilayer. Membrane lipid organization reflects a dynamic equilibrium of lipids moving across the bilayer in both directions. In this review, we summarize data supporting the role of specific membrane...