The lipoprotein lipase activity in the liver of neonatal (1 day old) rats was about 3 times that in the liver of adult rats. Perfusion of the neonatal liver with collagenase decreased the tissue-associated activity by 77%. When neonatal-rat liver cells were dispersed, hepatocyte-enriched (fraction I) and haemopoietic-cell-enriched (fraction II) populations were obtained. The lipoprotein lipase ...

isolation of pigmented t.violaceum from a patient with endothrix infection associated with the inflammatory and kerion symptoms and also isolation of the non- pigmented strain from another patient with non-inflammatory symptoms, led us to investigate the enzymic activities in both strains. most of the studies on dermatophyte compoxition have shown the relationship between proteolytic enzymes an...

The effect of castanospermine (CSTP), an inhibitor of glucosidase I, on processing, activity, and secretion of lipoprotein lipase was studied in 3T3-L1 adipocytes. Processing was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of endoglycosidase H (endo H)-digested subunits of lipoprotein lipase from cells incubated 1-2 h with [35S]methionine. Lipoprotein lipase...

conclusions the findings of this study indicated that these isolates were plant pathogenic fungi, which entered seawater from the environment, and were likely to have a suitable lipase activity on plant oils. results four fungal species were isolated from water samples of the caspian sea (north of iran) between february and june 2011. the nucleotide sequences reported for three of these isolate...

Abstract Lipase is an enzyme commonly applied as a catalyst in the food industry, synthesis of bioplastics, and production biodiesel. Candida rugosa lipase (CRL) one widely used lipases due to its high selectivity activity. However, operating temperatures can denature enzymes, affecting activity structural changes. An be immobilized on supporting matrix improve stability. Ethanol immobilization...

A gene encoding Rhizopus oryzae lipase containing prosequence (ProROL) was cloned into the pPICZαA and electrotransformed into the Pichia pastoris X-33 strain. The lipase was functionally expressed and secreted in Pichia pastoris with a molecular weight of 35 kDa. The maximum lipase activity of recombinant lipase (rProROL) was 21,000 U/mL, which was obtained in a fed-batch cultivation after 168...

The subcellular distribution in rat liver and rat kidney of the enzymes hydrolyzing triglycerides at acid pH has been studied. The lipase was shown to be lysosomal by the purification of lysosomes and by the isolation of Triton WR 1339fIlled liver lysosomes. The lipase was found to be bound to the lysosomal membranes, had a pH optimum of 4.2, and required Triton X-160 in the reaction mixture fo...

Pancreatic lipase requires colipase, a protein cofactor, to counteract the in vitro inhibition by bile salt. Lipase activity is inhibited by nonsteroidic detergents regardless of their charge and structure. Detergent-inhibited lipase is reactivated by colipase but in all cases activation is limited to a narrow range of detergent concentration. Complementary studies on the bile salt and detergen...

An extra-cellular lipase produced by Bacillus licheniformis MTCC 6824 was purified to homogeneity by ammonium sulphate fractionation, ethanol/ether precipitation, dialysis, followed by anion-exchange chromatography on Amberlite IRA 410 (Cl(-) form) and gel exclusion chromatography on Sephadex G 100 using Tris-HCl buffer (pH 8.0). The crude lipase extract had an activity of 41.7LU/ml of culture ...

The lingual serous glands of rat tongue secrete a potent lipase that hydrolyzes long chain triglycerides. The characteristics of this lipase were studied in preparations of lingual glands from Sprague-Dawley rats. Lipase activity was measured by the release of free fatty acids from emulsified tri[3H]oleate. Lipase activity in the 100,000 x g supernatant of lingual serous gland homogenate was ex...