Adenosine deaminase (ADA, EC 3.5.4.4) is a ubiquitous (beta/alpha)8-barrel enzyme crucial for purine metabolism and normal immune competence. In this study, it was observed that loss of enzyme activity of murine ADA (mADA) precedes the global secondary and tertiary structure transition when the protein is exposed to denaturant. The structural mechanism for this phenomenon was probed using site-...

Arvanil, a structural "hybrid" between the endogenous cannabinoid CB1 receptor ligand anandamide and capsaicin, is a potent agonist for the capsaicin receptor VR1 (vanilloid receptor type 1), inhibits the anandamide membrane transporter (AMT), and induces cannabimimetic responses in mice. Novel arvanil derivatives prepared by N-methylation, replacement of the amide with urea and thiourea moieti...

Drug development is originally carried out on a trial and error basis and it is cost-prohibitive. To minimize the trial and error risks, drug design is needed. One of the compound development processes to get a new drug is by designing a structure modification of the mother compound whose activities are recognized. A substitution of the mother compounds alters the physicochemical properties: li...

Gulf toadfish (Opsanus beta) use a unique pulsatile urea excretion mechanism that allows urea to be voided in large pulses via the periodic insertion or activation of a branchial urea transporter. The precise cellular and subcellular location of the facilitated diffusion mechanism(s) remains unclear. An in vitro basolateral membrane vesicle (BLMV) preparation was used to test the hypothesis tha...

SCHMIDT-NIELSEN, BODIL, AND CAROLYN RUTH SHRAUGER. Hundling of urea and related cumpuunds by the renal tubuks of the frog. Am. J. Physiol. PO&$: 483-488. 1963.-A high arginase activity is found in the kidney of the adult frog Ram catesbeiana which actively secretes urea. Urea accumulates in the kidney in concentrations around seven to eight times the blood concentration. To test whether the kid...

a liquid membrane containing diethylene glycol monobutyl ether (2) shows specificity for k ion in transport while diethylene glycol dibutyl ether (1) was selective for na over k . as an extractant, ligand 2 exhibited pronounced extraction selectivity for k over na in comparison with ligand 1

ROW-RAMEL, FRANCOISE, FRAN~OISE CHOMETY, AND G. PETERS. Urea concentrations in hh.darj?uid and in wnal tissue of nondiuretic rats. Am. J# Physiol. 2 15(Z) : 429-438. 1968.-Concentrations of urea and of inulin or polyfructosan were measured in proximal and distal tubular fluid of nondiuretic rats (P,,,, 5.5 =t 0.6 rn~) and rats infused with small doses of urea (P,,,, 11.8 + 1.1 mM>, Papillas and...

?Ur readers to form an opinion as o involved. They are not inconsiderab, Urea-Stibamine" has now a ;vell"^Stfnd the reputation in the treatment of kala-az , question of its supply and cost affects t ^ health Departments of four provinc caseApparently there were two issues i ^reahe first was as to the use of the na t^efamine the second the al|e|ad packed Ln,on Drug Co. was issuing the ciruj, f ^...

Here, new N,N′-dipyridoxyl(4,4''-diaminodiphenylether) Schiff-base ligand has been synthesized and characterized by IR, 1H NMR and mass spectrometry. Also, geometry optimization and theoretical assignment of IR spectrum of the ligand have been computed by employing density functional theory (DFT) method. Two pyridine rings and benzene rings in the bridge region are not in the same plane. The ca...

Sir,?In reply to Capt. T. D. W. Gillespie's inquiry in the Novtmber issue of the I. M. Q. as to the quantity of quinine urea hydrochloride to be injected hypodermically to produce local anaesthesia, I may say as follows: In the New York Medical Journal (1915, II, 708) Amster recommends quin-urea hydro as a local anaesthetic. He has performed 200 severe operations with a freshly prepared solutio...