Ten strains each of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were habituated to gentamicin by serial passage in antibiotic containing medium. Complete cross-resistance to streptomycin, neomycin, kanamycin, and tobramycin in a linear proportional fashion was demonstrated at all stages of habituation. Most strains of Staph. aureus and Ps. aeruginosa showed a greater inc...

Pseudomonas aeruginosa is one of the most important pathogens that causes nosocomial infections and shows high level of antibiotic resistance. Integrons are one of the transposable elements in bacteria and their role in antibiotic resistance has been well demonstrated. The aim of this study was a molecular characterization of the integron genes and the determination of the resistance or sensiti...

Aerobic gram-negative bacilli isolated from clinical specimens from 1 January to 31 December 1976 were tested for gentamicin and tobramycin resistance by standardized disk testing. For Pseudomonas isolates, gentamicin resistance was 17.1% and tobramycin resistance was 2.8%. For other gram-negative bacilli, gentamicin resistance was 5.5% and tobramycin resistance was 5.4%. Seventy-four patients ...

Pseudomonas aeruginosa strain GN315, producing kanamycin acetyltransferase, can transmit a plasmid determining resistance to kanamycin, amikacin (BB-K8), butirosin, tobramycin, and sisomicin, but not gentamicin, to other pseudomonas strains. This plasmid, pMG5, belongs to the same incompatibility group as plasmids determining gentamicin resistance in P. aeruginosa via gentamicin acetyltransfera...

Vitek 2 (bioMérieux, Inc., Durham, NC) is a widely used commercial antimicrobial susceptibility testing system. We compared MIC results obtained by Vitek 2 to those obtained by the Clinical and Laboratory Standards Institute (CLSI) broth microdilution (BMD) reference method for 134 staphylococcal and 84 enterococcal clinical isolates. Nineteen agents were evaluated, including all those availabl...

The turbidimetric responses of a strain of Escherichia coli K12 to gentamicin and tobramycin were investigated. Both agents showed antibacterial activity below the conventionally measured minimum inhibitory concentration, but exposure to such subinhibitory concentrations of either agent generated a bacterial population which was able to grow in previously inhibitory concentrations at a rate equ...

Enterococcus faecalis isolates were examined by an automated identification and susceptibility system. Almost all of the 97 isolates were ampicillin susceptible (n = 86) and tetracycline resistant (n = 89). All were nitrofurantoin susceptible. About a third of isolates showed high level resistance to the aminoglycosides streptomicin and gentamicin and this was usually associated with ciprofloxa...

The in vitro susceptibilities of 150 clinical isolates of Pseudomonas aeruginosa to the aminoglycoside tobramycin and eight other antimicrobials were evaluated. The Food and Drug Administration standardized disk diffusion method showed that tobramycin inhibited 100% of the 150 isolates with gentamicin inhibiting only 90.7%. The difference between colistin (97.3%) and tobramycin was less marked....

Jul 3, 2001. Vancomycin overview and pharmacokinetic calculations. 15. The peak is the measured drug concentration AFTER distribution. Vd or Volume of mg/dl will greatly elevate the calculated creatinine clearance. This is . (Use minimum SCr of 1 mg/dL.. (round dose to nearest 10 mg for gentamicin/ tobramycin and to nearest 50 mg for amikacin). A trough level should be obtained within 30 minute...

Background: This study aimed to investigate the occurrence of aminoglycoside resistance and the prevalence of the resistance-modifying enzyme genes, ant(3”)-III, ant(6’)-Ia, aac(6′)-Ie-aph(2”)-Ia, and aph(2’)-Id, in Enterococcus strains isolated in Kermanshah Province, west of Iran.Methods: In this cross-sectional study, 108 enterococcal isolates from urine, wound, blood, and cerebrospinal flui...