Fertility is dependent on a complex set of events, involving both male and female components. Normal sperm function involves many steps, including motility, capacitation, acrosome reactivity and, ultimately, fertilization of the oocyte. While male fertility is most often assessed by means of gross semen parameters, infertility may also be caused by abnormal sperm function, and only by performin...

Seminal plasma is a potential source of biomarkers for many disorders of the male reproductive system including male infertility. Knowledge of the peptide and protein components of seminal fluid is accumulating especially with the appearance of high-throughput MS-based techniques. Of special interest in the field of male infertility biomarkers, is the identification and characterization of diff...

Human seminal plasma contains a large array of proteins of clinical importance which are essentially needed to maintain the reproductive physiology of spermatozoa and for successful fertilization. Thus, isolation and identification of seminal plasma proteins is of paramount significance for their biophysical characterization and functional analysis in reproductive physiological processes. In th...

introduction: standard semen analysis is routinly used to evaluate the male fertility, however some researchers belive that sperm functional test should also be used with standard semen analysis for more precise evaluation of male fertility. the aim of this study was to consider the relation between different nuclear maturity tests and in vitro fertilization, in order to select the nuclear matu...

BACKGROUND Leukocytes represent the predominant source of reactive oxygen species both in seminal plasma and in sperm suspensions and have been demonstrated to negatively influence sperm function and fertilization rate in assisted reproduction procedures. Peroxidase test is the standard method recommended by WHO to detect semen leukocytes but it may be inaccurate. The aims of this study were (i...

background: sperm dna damage is an important factor in the etiology of male infertility. objective: the aim of the study was to evaluate the association of oxidative stress induced sperm dna damage with the pathogenesis of male infertility. materials and methods: the study comprised a total of 66 subjects, including fertile men (n=25) and infertile men (n=41) matched by age. seminal malondialde...

Background: Lipopolysaccharide (LPS) is a major component of Chlamydia spp, and of all Gram-negative bacteria. The role of LPS in alteration of sperm parameters is controversial. In this study quantification of Chlamydia-specific and general lipopolysaccharide in semen was correlated to semen quality. Materials and Methods: 255 male partners of infertile couples were recruited to produce urine ...

Infertility is a common yet complex problem affecting approximately 10-15% of couples attempting to conceive a baby. Especially, 40-50% of these factors are known as male-related disorders. Unlike female infertility, the cause of which is often easily identified, diagnosing male factors can be difficult. Male infertility is due to low sperm production, abnormal sperm function or blockages of sp...

sperm chromatin integrity has been being recognized as an important factor in male fertility. during normal fertilization, high quality sperm with intact chromatin are selected through natural selection in journey from vagina to fallopian tube. however, using assisted reproductive techniques, particularly icsi, the natural selection is bypassed. therefore sperm with dna breakage have the opport...