Thailand is considered as a non-endemic area for leishmaniasis. We report the first case of visceral leishmaniasis caused by Leishmania infantum in a Thai man living in Bangkok.

A new liquid culture medium prepared with chemicals that can be obtained economically and commercially was tested in in vitro cultivation of Leishmania promastigotes to obtain a large number of organisms to use in serological studies. The number of Leishmania infantum and Leishmania tropica promastigotes taken from Novy-MacNeal-Nicolle (NNN) medium reached 1 x 10(7)/ml at the end of the 8th day...

background: pentavalent antimonials are still the first choice treatment for leishmaniasis, but with low efficacy and resistance is emerging. in the present study, the effect of meglumine antimoniate (ma, glucantime) combined with paromomy-cin, miltefosine or allopurinol on in vitro susceptibility of leishmania tropica resistant isolate was evaluated. method : the drugs were obtained from comme...

a 5-month old puppy with muco-cutaneous lesions in the chin, around lips and eyes was exam­ined physically and microscopically for leishmaniasis. muco-cutaneous lesions containing a large num­ber of amastigotes of leishmania spp. were observed . amastigotes were also detected in liver and spleen of the puppy. the animal was positive with dipstick rk39 kit and high level of anti- leishmania anti...

b a ckground: the objective of this study was to assess the epidemiological characteristics of a new emerging focus of cutaneous leishmaniasis (cl) in southern villages of bam district, southeastern iran, 2010. m e t hods: a house-to- house census survey of 5544 individuals were interviewed and physically examined for the presence of active lesions or scars. diagnosis was confirmed by direct sm...

OBJECTIVE To evaluate the leishmanicidal activity of saponin, dasyscyphin C of Eclipta prostrata and sapogenin, gymnemagenol from Gymnema sylvestre leaves under in vitro conditions. MATERIALS AND METHODS Dasyscyphin C/Gymnemagenol were dissolved in phosphate buffered saline (PBS) and diluted with liquid medium to obtain concentrations ranging from 1000 to 15 mug /ml. The leishmanicidal activi...

BACKGROUND A rare variant of Leishmaniasis is Localized Leishmania Lymphadenitis which has been occasionally reported from south-eastern parts of Iran. So far, no molecular assay has been performed for diagnosing this variety of Leishmaniasis. METHODS Nineteen lymph node paraffin blocks were collected from 1994 to 2007. Parasite load count and histopathological patterns reported on Hematoxyli...

OBJECTIVES Leishmania can lead to a broad spectrum of diseases, collectively known as leishmaniasis. The A2 gene/ protein family could be one of the most eligible candidate factors of virulence in visceral leishmaniasis (VL). The previous results confirmed that in Leishmania infantum, several A2 proteins are abundantly expressed by the amastigote, but not the promastigote stage. As there are no...

We used the cysteine proteinase B (cpb) gene family of the trypanosomatid genus Leishmania as a target to develop rapid, specific, and easy-to-use polymerase chain reaction (PCR) tests to discriminate Leishmania infantum, Leishmania donovani, Leishmania tropica, Leishmania aethiopica, and Leishmania major. Identification of all 5 Old World species and validation of intraspecies variability are ...