The centromere regions of each chromosome in the complement of Nigella damascena (2n equals 2x equals 12) stain differentially with Giemsa at interphase and throughout all the principal stages of mitosis and meiosis. Each centromere is seen to consist of a pair of sister half-centromeres which appear as 2 differentially stained dots. The appearance and behaviour of these dots indicates that the...

One hundred years ago, Giemsa's stain was employed for the first time for malaria diagnosis. Giemsa staining continues to be the method of choice in most malarious countries, although, in the recent past, several alternatives have been developed that exhibit some advantages. Considerable progress has been made with fluorescent dyes, particularly with Acridine Orange (AO). The literature on the ...

We present a miniaturized centrifugal platform that uses density centrifugation for separation and analysis of biological components in small volume samples (~5 μL). We demonstrate the ability to enrich leukocytes for on-disk visualization via microscopy, as well as recovery of viable cells from each of the gradient partitions. In addition, we simplified the traditional Modified Wright-Giemsa s...

The possibility of preparing a permanent staining solution from the formulas for Nocht's (I) stain was suggested by the constant use of Jenner's (2) stain for routine blood examinations and malarial staining during the summer and fall months of ~9ox. In malarial blood the pigmented bodies of tertian fever and the crescent bodies of mstivo-autumnal fever are readily demonstrated with Jenner's st...

Malaria is a leading cause of morbidity and mortality worldwide. Prompt diagnosis and treatment are critical factors in reducing morbidity and mortality, as delayed treatment of malaria increases the risk of death. Microscopy has long been the standard for malaria diagnosis. Diagnosis plays a vital role in early detection of malaria which is an essential prerequisite for control of the disease....

A new direct immunofluorescence monoclonal antibody (DFA) method (Genetic Systems, Inc., Seattle, Wash.) for identification of Pneumocystis carinii in induced sputum and bronchoalveolar lavage specimens was compared in a blinded study with an established Giemsa stain method. We evaluated 148 consecutive clinical specimens from 104 patients with the following results. For the 67 patients (64%) i...

BACKGROUND Flow cytometry has come to occupy the vanguard of the high through put diagnostic techniques that have been used to differentiate between various chronic lymphoproliferative disorders (CLPD). However, economic considerations have created the need for minimal consensus panels that can yield maximum information at reasonable costs. AIMS To collect, analyse and correlate the morpholog...

The intravenous injection of C1498 cells into syngeneic or congenic mice has been performed since 1941. These injections result in the development of acute leukemia. However, the nature of this disease has not been well documented in the literature. Here, we provide a technical protocol for characterizing C1498 cells in vitro and for determining the nature of the induced leukemia in vivo. The f...