Toxoplasma gondii matrix antigen 1 (TgMAG1), known as the 65-kDa protein, which is abundantly expressed in both bradyzoites and tachyzoites, was evaluated as a candidate for the development of a diagnostic reagent for ovine toxoplasmosis. The TgMAG1 gene was expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST), and the recombinant TgMAG1 (rTgMAG1) was tested in...

A semi-quantitative latex agglutination test for bovine serum CRP levels has been established by mixing diluted serum (or diluted standard serum) with a 1% latex suspension containing 0.489 #m latex particles coated with affinity-purified antibody at a ratio of 20 #g/mg latex. The agglutination was performed on a glass slide in a moist chamber at room temperature with 45 min. incubation. This t...

The latex agglutination card test (Rubascan) for the detection of rubella antibody was compared with the standard hemagglutination inhibition and enzyme-linked immunosorbent assay tests. There was complete agreement with sera which had hemagglutination inhibition titers of >/=16. Sera with low levels of antibody which were positive in the enzyme-linked immunosorbent assay, however, gave negativ...

A latex slide test is described which is suitable for handling large numbers of specimens. The buffered latex suspension used is easily prepared from stable stock solutions at relatively low cost. It is not necessary to add Cohn fraction II (gamma globulin) to the latex reagent. A comparison has been made with the sheep cell agglutination (Rose-Waaler) test in 508 cases and a 96% overall agreem...

Morton, Harry E. (University of Pennsylvania, Philadelphia). Mycoplasma-latex agglutination reaction. J. Bacteriol. 92:1196-1205. 1966.-The building up of Mycoplasma cell mass through adsorption to carrier particles as a method for enhancing the agglutination reaction to identify Mycoplasma is described. Mycoplasma cells of human, avian, swine, goat, sewage, and tissue-culture origin were adsor...

A latex agglutination test for the detection of Pseudomonas pseudomallei antigen in urine was evaluated for the rapid diagnosis of melioidosis. With unconcentrated urine, antigen was detected in only 18% of patients with melioidosis overall. However, when urine was concentrated 100-fold, antigen was detected in 47% overall and in 67% of patients with septicaemia or disseminated infection, in wh...

A British polystyrene latex-bovine gamma globulin fixation test which can be used for serum titrations is described. It is technically easier to perform than the latex fixation test of Singer and Plotz (1956) and appears to be equally sensitive. There was agreement with a sensitized sheep cell agglutination test in 264 of 300 cases (88%).

The aim of this study was to determine if the absence of circulating D-dimers, as determined by latex agglutination assays, can correctly exclude the presence of pulmonary embolism using pulmonary angiography as the diagnostic endpoint. Blood samples were obtained prospectively at the time of angiography for suspicion of acute pulmonary embolism. Plasma was assayed for D-dimer by five different...

SYNOPSIS Fibrinogen antigens were measured either with an agglutination inhibition method (using latex particles coated with fibrinogen; Diagen test) or with a direct agglutination technique (using latex particles coated with a mixture of anti-D and anti-E antibodies; Thrombo-Wellcotest). Both methods were compared with the tanned red cell haemagglutination inhibition immunoassay (TRCHII) durin...