Lactobacillus strains with the potential of protecting fresh dairy products from spoilage were studied. Metabolism and antifungal activity different L. plantarum, brevis, sakei strains, isolated Sardinian meat products, assessed. The metabolite fingerprint each strain was obtained by GC-MS data submitted to multivariate statistical analysis. discriminant analysis correctly classified samples sp...

Continuous operation of alcohol dehydrogenase (ADH) catalysed enantioselective reduction in a biphasic sytem showed gains in productivity and stability of the overall reaction system. The total turnover numbers obtained for the cofactor NADP + are high with up to 1.5 10 4 . Productivity for (R)-2-butanol with Lactobacillus brevis ADH was up to 26 kmol (mol enzyme) 1 . Enantioselectivity was gre...

Background and Objective: The aim of this study was to compare the cytotoxic effects of local probiotic bacteria, including Lactobacillus paracasei, Lactobacillus brevis, while isolated from “Tarkhine” food and the induction of apoptosis in the HT–29 human colon adenocarcinoma cell line and normal fibroblasts. Methods: HT–29 and L–929 cell lines were trea...

Various foods may be used to deliver probiotic bacteria into the gastrointestinal tract; one such example is Requeijão, a Portuguese whey cheese. Survival and stability of Bifidobacterium animalis strains BLC-1, Bb-12, and Bo, Lactobacillus acidophilus strains LAC-1 and Ki, L. paracasei ssp. paracasei strain LCS-1 and L. brevis strain LMG 6906 inoculated into Requeijão, when exposed to simulate...

The spoilage of beer by bacteria is of great concern to the brewer as this can lead to turbidity and abnormal flavors. The polymerase chain reaction (PCR) method for detection of beer-spoilage bacteria is highly specific and provides results much faster than traditional microbiology techniques. However, one of the drawbacks is the inability to differentiate between live and dead cells. In this ...

Corresponding Author: Jian Mao, State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China Tel: +86-510-8532-9062, Fax: +86-510-8591-2155, E-mail: [email protected] Abstract: Previous studies showed that γ-aminobutyric acid (GABA) can protect some GABA-producing bacteria against acid stress through glutamate decarboxylat...

BACKGROUND AND PURPOSE Twenty lactobacilli isolated from vaginal samples of healthy volunteers were characterized according to polyphasic taxonomy. A broad spectrum of activity and protective properties of these vaginal isolates has been reported. METHODS Phenotypic and genotypic methods such as random amplified polymorphic DNA (RAPD), species-specific and BOX polymerase chain reaction (PCR),...

Market fresh makgeolli was stored at different temperatures of 4°C and 25°C to assess the change of the microbial diversity according to the storage temperature and period. Yeast counts increased until day 3 of storage and decreased thereafter. General and lactic acid bacterial counts continuously increased during storage. The data indicated that the control of growth of microorganisms, particu...

Lactobacillus brevis accumulates lactose and nonmetabolizable lactose analogues via sugar/H+ symport, but addition of glucose to the extracellular medium results in rapid efflux of the free sugar from the cells due to the uncoupling of sugar transport from proton transport. By using vesicles of L. brevis cells, we recently showed that these regulatory/effects could be attributed to the metaboli...