This study aimed to evaluate the effect of short-term forage substitution with ensiled olive cake (OC), on yield, composition and fatty acid (FA) profile cows’ milk. Mid-lactating Holstein-Friesian cows were randomly assigned for 21 days two isoenergetic isoproteic feeding treatments (12 animals per treatment), containing 0 10% DM OC (C groups, respectively). Milk yield was recorded daily, milk...

BACKGROUND Subclinical mastitis is of concern in veterinary hospitals because contagious mastitis pathogens might be unknowingly transmitted to susceptible cows and then back to their farm of origin. OBJECTIVES To evaluate the California mastitis test (CMT) as an indicator of intramammary infection (IMI) in lactating dairy cows admitted to a veterinary hospital. ANIMALS A total of 139 admis...

Butyrophilin is a glycoprotein expressed on the apical surfaces of secretory cells in lactating mammary tissue, which may function in the secretion of milk-fat droplets (Franke, W. W., Heid, H. W., Grund, C., Winter, S., Freudenstein, C., Schmid, E., Jarasch, E.-D., and Keenan, T. W. (1981) J. Cell Biol. 89, 485-494). A cDNA clone encoding bovine butyrophilin was isolated and the primary struct...

Methane emission factors are lacking from different livestock species in various production states fed varying diets. The objectives of the present study were to measure effects of physiological state, silage type, and supplemental NDF on DMI, DM digestibility (DMD), and methane emissions of lactating and dry dairy cows using two 4 × 4 Latin squares (5-wk periods) with four lactating (114 DIM ±...

There has been a significant decline in the reproductive performance of dairy cattle in recent decades. Cows, take longer time to return to the oestrus after calving, have poorer conception rates, and show fewer signs of oestrus. Achieving good reproductive performance is an increasing challenge for the dairy producer. In this study we focus on understanding the overall biological phenomena ass...

The establishment and maintenance of pregnancy in lactating dairy cows is a complex biological event that is influenced by a multitude of factors, from the reproductive biology of the cow to managerial aspects of the dairy farm. It is often mentioned in the scientific literature that fertility in dairy cows has declined concurrent with major advances in milk production. Some of this decline is ...

Activity of acetyl-CoA carboxylase (ACC)-alpha is rate limiting for de novo synthesis of fatty acids. The encoding gene is expressed by three different promoters. We characterized promoter III (PIII) from cow, previously only known from sheep. Quantitation of transcripts by RNAse protection assays and real time PCR revealed that PIII is primarily expressed and strongly induced ( approximately 2...

The objective of this trial was to measure performance of paired pens of lactating Jersey and Jersey-Holstein crossbred (JX) vs. Holstein (H) cows over a year in a Wisconsin confinement dairy herd. Average daily milk yield of JX was 5.5 kg/ cow less than H, whereas average milk fat and true protein percentages were 0.61 (4.26 vs. 3.65%) and 0.19 (3.05 vs. 2.86%) percentage units, respectively, ...