A lactate clamp method has been developed to quantify the whole body lactate utilization in conscious, unstressed rats. Dichloroacetate (DCA), a known lactate utilization enhancer, was used to validate the method. Fasting blood lactate concentrations before the clamps were identical for DCA-treated (1 mmol/kg) and control groups (1.65 ± 0.37 vs. 1.65 ± 0.19 mM). The animals received a primed co...

objective(s): many cancer cells show significant resistance to drugs that kill drug sensitive cancer cells and non-tumor cells and such resistance might be a consequence of the difference in metabolism. therefore, studying the metabolism of drug resistant cancer cells and comparison with drug sensitive and normal cell lines is the objective of this research. material and methods:metabolism of c...

Chemical Labeling has been employed to attempt to identify the lactate transport protein of rabbit erythrocytes, which have a very high capacity for stereoselective lactate transport. The lactate transport protein catalyzes lactate/proton cotransport (or lactate/hydroxyl exchange) at physiological pH, as demonstrated by uphill proton fluxes induced by lactate gradients. However, lactate/lactate...

Abstract Staphylococcus aureus (S. aureus) is a leading cause of prosthetic joint infection (PJI) typified by biofilm formation. Our laboratory has identified preferential myeloid-derived suppressor cell (MDSC) recruitment as critical mechanism for persistence, MDSCs are main source IL-10. We screened the Nebraska Transposon Mutant Library to identify S. mutants impaired in their ability trigge...

It is generally acknowledged that calcium lactate (ca-lactate) fortification of soymilk to achieve the equivalent level in cow’s milk (1.2 mg/g) causes instability soymilk. In order produce acceptable calcium-enriched with great stability, three different kinds chelating agents were added, namely tripotassium citrate (TPC), trisodium (TSC), and sodium hexametaphosphate (SHMP). this study, addit...