The tinetics of NADH, 3-thionicotinamide adenine dinucleotide (TNAD), and oxamate binding to the H4 isoenzyme of lactate dehydrogenase from the pig has been investigated by temperature jump techniques. The dissociation rate constant for TNAD is considerably larger than for NADH, whereas the recombination rate constant is smaller for the oxidized coenzyme than for the reduced molecule. The kinet...

Escherichia coli strains (KJ060 and KJ073) that were previously developed for succinate production have now been modified for malate production. Many unexpected changes were observed during this investigation. The initial strategy of deleting fumarase isoenzymes was ineffective, and succinate continued to accumulate. Surprisingly, a mutation in fumarate reductase alone was sufficient to redirec...

Pulmonary microvascular endothelial cells possess both highly proliferative and angiogenic capacities, yet it is unclear how these cells sustain the metabolic requirements essential for such growth. Rapidly proliferating cells rely on aerobic glycolysis to sustain growth, which is characterized by glucose consumption, glucose fermentation to lactate, and lactic acidosis, all in the presence of ...