The additional lactate dehydrogenase isozyme from rabbit testis and sperm, lactate dehydrogenase isozyme-1 (B4) from heart, and lactate dehydrogenase isozyme-5 (A4) from liver have been separated and partially purified by preparative starch gel electrophoresis. Studies performed on these fractions comprised K, and optimum substrate concentration for pyruvate, a-ketobutyrate, and lactate, inhibi...

Activity of L-threonine aldolase in rat liver cytosolic extract was not affected by the omission of alcohol dehydrogenase in a previously established NADPH-linked alcohol dehydrogenase-coupled assay. The liver extract was able to catalyse the dehydrogenation of NADPH with either acetaldehyde (a product of L-threonine aldolase action) or 2-oxobutyrate (a product of L-threonine dehydratase action...

A common problem in clinical medicine is the diagnosis of acute chest pain. Usually, the history, clinical findings, and electrocardiographic evidence are sufficient to enable the clinician to reach a confident diagnosis. Some cases, however, have atypical features or show an electrocardiogram difficult of interpretation, because of previous infarction, left bundle-branch block, previous left v...

Activity of total lactate dehydrogenase (LDH) and that of the heat-stable isozyme (LDH-1) was measured by the method of Strandjord and Clayson in the serum of 62 cardiac patients with congenital and valvular heart disease before and after cardiac catheterization and at follow-up examination after cardiac surgery. A universal elevation of both total and heat-stable LDH was found in patients with...

Background: Lactate dehydrogenase (LDH) is an intracellular enzyme found in cells almost all organ systems, which catalyzes the interconversion of pyruvate and lactate, LDH has been proved to be a prognostic factor with high accuracy diseases involving multiple injuries such as acute heart failure (AHF) severe pancreatitis (AP), potential biomarker patients COVID-19. Patients methods: This stud...

OBJECTIVE Wharton jelly-derived mesenchymal stem cells (WJMSCs) exhibit multilineage differentiation potential and can be used to treat multiple organs. However, diabetes affects the repair capability of MSCs. The aim of this study was to evaluate the effect of diabetic patient-derived serum on WJMSC behavior. METHODS WJMSCs at passage 3 were treated with serum derived from type 2 diabetic pa...

The protozoan parasite Cryptosporidium parvum causes waterborne diseases worldwide. There is no effective therapy for C. parvum infection. The parasite depends mainly on glycolysis for energy production. Lactate dehydrogenase is a major regulator of glycolysis. This paper describes the biochemical characterization of C. parvum lactate dehydrogenase and high resolution crystal structures of the ...

The MJ0490 gene, one of the only two genes of Methanococcus jannaschii showing sequence similarity to the lactate/malate family of dehydrogenases, was classified initially as coding for a putative l-lactate dehydrogenase (LDH). It has been re-classified as a malate dehydrogenase (MDH) gene, because it shows significant sequence similarity to MT0188, MDH II from Methanobacterium thermoautotrophi...

We investigated the mechanism of cancer-associated fibroblasts (CAFs) in promoting the invasion and metastasis of pancreatic cancer cells in a non-vascular manner. We verified the original generation of isolated cultured CAFs and normal fibroblasts (NFs) based on the expression of α-SMA and vimentin, and we examined the cell glycolysis level through glucose consumption and lactate production ex...

1. The interconversion of hydroxypyruvate and l-glycerate in the presence of NAD and rat-liver l-lactate dehydrogenase has been demonstrated. Michaelis constants for these substrates together with an equilibrium constant have been determined and compared with those for pyruvate and l-lactate. 2. The presence of d-glycerate dehydrogenase in rat liver has been confirmed and the enzyme has been pu...