Histone deacetylase inhibitors (HDIs) and HAMLET (human alpha-lactalbumin made lethal to tumor cells) interact with histones, modify the structure of chromatin, and trigger tumor cell death. This study investigated how the combination of HDIs and HAMLET influences cell viability, histone acetylation, and DNA integrity. The pretreatment of tumor cells with HDIs was shown to enhance the lethal ef...

Abstract In eusocial honey bees, Apis mellifera , diet, gut microbiota and nestmates can all contribute to the health of freshly emerged individual workers, but their relative importance for longevity body weight is currently unknown. Here, we show that diet most relevant, followed by presence nestmates. Freshly workers were randomly assigned eight treatments (with or without honey/pollen, prot...

The low-frequency (1-200 cm(-1)) vibrational spectra of peptides and proteins in solution have been investigated with ultrafast optical heterodyne-detected Raman-induced Kerr-effect spectroscopy (OHD-RIKES). Spectra have been obtained for di-L-alanine (ALA(2)) and the alpha-helical peptide poly-L-alanine (PLA) in dichloroacetic acid solution. The poly-L-alanine spectrum shows extra amplitude co...

Background Atopic dermatitis is a multifactorial disease associated with elevated production of IgE and sensitization to multiple food and inhalant allergens.The aim of this study was to assess the levels of total serum IgE (IgE) and specific IgE (sp IgE ) for Dermatophagoides pteronyssinus (Dp), Blomia tropicalis (Bt), egg white, casein, alpha-lactalbumin and beta-lactoglobulin in patients wit...

Protein structure analysis from DNA sequences is an important and fast growing area in both computer science and biochemistry. One of the most important problems is that two proteins, both of which have the similar three-dimensional structure, have different functions, such as ll/sozyme and lactalbumin. In such cases, comparative analysis of both amino acid sequences is effective to detect the ...

The epithelial cells from mammary glands of mature virgin mice synthesize increasing levels of casein and cY-lactalbumin when cultured in the presence of insulin, hydrocortisone, and prolactin. When DNA synthesis is blocked in these cultures by the addition of cytosine arabinoside or 5-fluorodeoxyuridine, augmented milk-protein production does not occur. The effects of cytosine arabinoside are ...

In order to investigate the effects of collagen peptide ingestion on fibroblasts and the extracellular matrix in the dermis, collagen peptide was administered orally to pigs at 0.2 g/kg body weight/d for 62 d, and its effects were compared with those of lactalbumin and water controls. Fibroblast density, and diameter and density of collagen fibrils were significantly larger in the collagen pept...

The denaturation of ribonuclease A, lysozyme alpha-lactalbumin, and myoglobin by urea, guanidine hydrochloride, and guanidine thiocyanate has been followed with the use of difference spectral measurements. The free energy of stabilization (delta GH2OD) has been determined by the linear extrapolation of the free energy of denaturation to zero denaturant concentration. The values of delta GH2OD a...

Cell-surface galactosyltransferase was studied in suspensions of intact baby hamster kidney fibroblasts with both endogenous and exogenous glycoprotein acceptors. The cell-surface location of galactosyltransferase was demonstrated in experiments with the enzyme modifier alpha-lactalbumin, which does not enter the cell. The addition of alpha-lactalbumin to the assay medium for galactosyltransfer...