alterations in the expression of micrornas (mirnas) have been proposed to play a role in the pathogenesis of acute lymphoblastic leukemia (all) and chronic lymphocytic leukemia (cll). dicer is one of the main regulators of mirna biogenesis, and deregulation of its expression has been indicated as a possible cause of mirna alterations observed in various cancers. our aim was to analyze the expre...

the present study was aimed to isolate and characterize the lipolytic enzyme producing bacteria from soil samples of regions around zayande-rood river of isfahan, iran. soil samples were collected from 15 cm depth of soil surface. based on morphology, distinct colonies were isolated and purified through streak culture on to standard agar plates. isolated colonies were examined for lipase activi...

background: trichomonas vaginalis causes trichomoniasis and metronidazole is its chosen drug for treatment. ferredoxin has role in electron transport and carbohydrate metabolism and the conversion of an inactive form of metronidazole (co) to its active form (cpr). ferredoxin gene mutations reduce gene expression and increase its resistance to metronidazole. in this study, the frequency of ferre...

background: oculocutaneous albinism type1 (oca1) is characterized by the absence of melanin pigmentation. the muta­tion on tyr gene makes oca1 as an autosomal recessive genetic disorder. in this study, we delineated the genetic analysis of an iranian family with four members affected with oca1.  methods: clinical exams and paraclinical test were performed for all patients of the case family, al...

background: β-thalassemia as a hereditary disease is defined as defective synthesis of   β-globin chains, resulting in erythropoiesis abnormalities and severe anemia. different studies have shown that cytokines and cytokine gene polymorphisms play a major role in the pathogenesis of   β-thalassemia. single nucleotide polymorphisms (snps) within the promoter region or other regulatory sequences ...

objective: β-thalassemia is caused by absence or reduction of β-globin chain synthesis. one of the effective therapeutic methods for this disease can be gene therapy by viral vectors. the capacity of lentiviral vectors is approximately 8 kb, we designed a 6 kb construct containing mini lcr and β-globin gene instead of lcr region. the aim of this study is to make a recombinant lentiviruses conta...

background attention deficit hyperactivity disorder (adhd) is a multi-factorial disorder that has defined by hyperactivity, impulsivity and attention deficits. various neurotransmitters such as dopamine can play a role in its pathophysiology. the aim of this study was to examine the association of two common single nucleotide polymorphisms in drd2 gene, taq i a (t/c) and taq i b (g/a), with adh...

background: the sry gene (sry) provides instructions for making a transcription factor called the sex-determining region y protein. the sex-determining region y protein causes a fetus to develop as a male. in this study, sry of 15 spices included of human, chimpanzee, dog, pig, rat, cattle, buffalo, goat, sheep, horse, zebra, frog, urial, dolphin and killer whale were used for determine of bioi...

background: the multifunctional transforming growth factor beta (tgf-β) is a glycoprotein that exists in three isoforms. tgf-β3 expression increases in fetal wound healing and reduces fibronectin and collagen i and iii deposition, and also improves the architecture of the neodermis which is a combination of blood vessels and connective tissue during wound healing. fibroblasts are key cells in t...

quantitative traits locus for milk production traits has been described on centromeric end of bovine chromosome 14. reports name the acyl coa: diacylglycerol acyltransferase (dgat1) gene as a potential candidate gene with dinucleotide substitution (aa to gc) in exon viii which causes the change of lysine to alanine in amino acid (k232a).the aim of the present study was to estimate the frequency...