Patients and methods 34 patients were selected on clinical grounds by members of the French reference center for autoinflammatory diseases (CeReMAI). 9 of them were considered positive control samples as they had previously known variants (by Sanger sequencing) and were run in parallel for methodological validation. A custom panel of 108kb was designed to capture the genomic regions of interest...

We introduce the first commercially available comet assay for the detection and quantification of DNA damage in individual eukaryotic cells. The major difficulty of the comet assay is the preparation of the slides needed to immobilize the samples throughout the lysis and electrophoretic procedures. The CometAssay kit uses a proprietary technology to precoat glass microscope slides to allow dire...

Multilocus sequence typing (MLST) of Salmonella is useful method for replacing serotyping using antisera but is limited by difficulties associated with in polymerase chain reaction (PCR). We optimized the PCR reaction, especially annealing temperature and extension time (94°C for 2 min; 40 cycles at 94°C for 30 sec, 56.8°C for 1 min, 72°C for 2 min; and 72°C for 10 min). The degradation of PCR ...

II. General Guidance for Evaluation of Pathogen Test Kit Performance 1. Purpose, Scope and Audience 2. General Considerations 3. Inoculum Number of strains Strain selection (Table 1) Preparation of inoculum Inoculation of matrix 4. Matrix Choice of matrix (Tables 2a, Table 2b) Decision Criteria Illustrating When New Validation Studies Should be Conducted for an Existing Method Characterization ...

T hrough its Priority Review mechanism, the U.S. Food and Drug Administration (FDA) recently approved 2 radiopharmaceutical products for use as diagnostic PET agents. The first, approved on May 27, was Axumin (18F-fluciclovine) injection, indicated for use in PET imaging to identify suspected sites of prostate cancer recurrence in men with elevated levels of prostate-specific antigen (PSA) foll...

introduction: neurotensin (nt) is a tridecapeptide that binds specifically to neurotensin receptors. several forms of cancer, including small cell lung cancer, colon, pancreatic and prostate carcinomas especially exocrine pancreatic carcinomas express receptors for neurotensin peptide. radiolabeled neurotensin derivatives with a high affinity for these receptors might be used for scintigraphy. ...

The Illumina® Nextera® XT DNA Library Preparation Kit workflow was automated on PIPETMAX®. For comparison, 12 technical replicate libraries were prepared side by side, using manual liquid handling or automated on PIPETMAX. The 24 libraries were pooled and sequenced on an Illumina MiSeq® sequencing system. Both library preparation methods generated high quality data with >95% mapped reads and op...

The c-kit protein plays a major role in the regulation of germ cell development. Its expression and distribution in rodent testes have been widely reported. However, research regarding c-kit expression in domestic animals is scarce, and the expression pattern and distribution of c-kit in germ cells have not been clearly defined. In this study, a specific antigenic region for goat c-kit was desi...

A lipiodol solution of (188)Re-4-hexadecyl-2,2,9,9-tetramethyl-4,7-diaza-1,10-decanedithiol ((188)Re-HDD/lipiodol) is in clinical study for liver cancer therapy. However, formulation of it is difficult due to highly active and unstable sulfhydryl groups. We produced new kits using diacetylated HDD (AHDD), in which sulfhydryl groups are protected. We found that AHDD kit can replace HDD kit due t...