the composite diazotization coupling reaction of sulfanilic acid and n, ndimethylaniline was used for the precise, accurate, and rapid determination of trace amounts of nitrite ion (0.02-3 µg/ml). the reaction was monitored spectrophotometrically by measuring the absorbance of the produced methyl orange at 510 nm by a fixed the method of 5.0 minutes. the relative standard deviation for the dete...

In this study, a new reaction system for quantitative determination of ascorbic acid was introduced. The developed method is based on inhibitory effect of ascorbic acid on the Orange G-bromate system. The change in absorbance was followed spectrophotometrically at 478 nm. The dependence of sensitivity on the reaction variables including reagents concentration, temperature and time was investiga...

Attempts to obtain experimental values for the kinetic parameters of phenanthridine oxidation by guinea pig or rabbit liver aldehyde oxidase using common spectrophotometric methods have not been successful due to a lower limit of detection. In the present study, a new spectrofluorimetric assay in combination with a multivariate calibration method for enzymatic kinetic study of aldehyde oxidase ...

Attempts to obtain experimental values for the kinetic parameters of phenanthridine oxidation by guinea pig or rabbit liver aldehyde oxidase using common spectrophotometric methods have not been successful due to a lower limit of detection. In the present study, a new spectrofluorimetric assay in combination with a multivariate calibration method for enzymatic kinetic study of aldehyde oxidase ...

a simple and sensitive extraction-spectrophotometric method for rapid and accurate determination of benzocaine in pure and dosage forms was developed. benzocaine was effectively extracted as a 1:1 ion pair complex with dicyclohexyl-18-crown-6 and calmagite in acidic media into chloroform, followed by spectrophotometric determination at 486 nm. molar absorptivity of the ternary complex at this w...