Adenosine triphosphate-sensitive K(+) (KATP) channels play an essential role in glucose-induced insulin secretion from pancreatic β-cells. It was recently reported that the KATP channel is also found in the enteroendocrine K-cells and L-cells that secrete glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1), respectively. In the present study, we investigated t...

The mechanism by which ATP-sensitive K+ (KATP) channels open in the presence of inhibitory concentrations of ATP remains unknown. Herein, using a four-state kinetic model, we found that the nucleotide diphosphate UDP directed cardiac KATP channels to operate within intraburst transitions. These transitions are not targeted by ATP, nor the structurally unrelated sulfonylurea glyburide, which inh...

ATP-sensitive potassium (KATP) channels link membrane excitability to metabolic state to regulate a series of biological activities including the vascular tone. However, their ability to influence hypertension is controversial. Here we aim to investigate possible alteration of KATP channel in vascular smooth muscles (VSMs) during hypertension development process. In this study, we used 16-week-...

Diosgenin is a natural steroid sourced from plants and exhibits good analgesic anti-inflammatory activity. However, mechanism of action associated with the activity diosgenin not yet delineated. Hence, in present study, we explored on nitric oxide/cyclic guanosine monophosphate/adenosine monophosphate sensitive potassium channels (NO/cGMP/kATP). The role (NO/cGMP/kATP) Wistar rat was studied us...

Substantial evidence has shown that obstructive jaundice can induce vascular hyporesponsiveness. The present study was designed to investigate mechanisms of MaxiK channel and KATP underlying cholestasis-induced vascular dysfunction. The isolated thoracic aorta was used to explore norepinephrine (NE)-induced contraction. The function of MaxiK and KATP channels were investigated using whole-cell ...

Nguyen, Thien-Son, H. Richard Winn, and Damir Janigro. ATP-sensitive potassium channels may participate in the coupling of neuronal activity and cerebrovascular tone. Am. J. Physiol. Heart Circ. Physiol. 278: H878–H885, 2000.—K1 dilate and constrict cerebral vessels in a dosedependent fashion. Modest elevations of abluminal K1 cause vasodilatation, whereas larger extracellular K1 concentration ...

Pék-Scott, Marta, and Peter L. Lutz. ATP-sensitive K1 channel activation provides transient protection to the anoxic turtle brain. Am. J. Physiol. 275 (Regulatory Integrative Comp. Physiol. 44): R2023–R2027, 1998.—There is wide speculation that ATP-sensitive K1 (KATP) channels serve a protective function in the mammalian brain, being activated during periods of energy failure. The aim of the pr...

Endothelial dysfunction is commonly observed in patients with hypertension, type II diabetes mellitus, and hypercholesteremia and is considered to be an early event in the changes accompanying atherosclerosis [1,2]. Disruption of normal endothelial function leads to loss of vasomotor control, reduced production of nitric oxide (NO), formation of a procoagulant surface, and promotion of inflamma...

In the present study, we have investigated the mechanism underlying the activation by 5-amino-N-[2-(2-chlorophenyl)ethyl]-N'-cyano-3- pyridinecarboxamidine (KRN4884), a new K+ channel opener, of ATP-sensitive K+ (KATP) channels in single ventricular cells of guinea pig hearts by the inside-out patch-clamp method. In the presence of intracellular ATP (1 mM), KRN4884 (0.1-3 microM) activated KATP...

ATP-sensitive potassium (KATP) channels link cell metabolism to membrane excitability and are involved in a wide range of physiological processes including hormone secretion, control of vascular tone, and protection of cardiac and neuronal cells against ischemic injuries. In pancreatic β-cells, KATP channels play a key role in glucose-stimulated insulin secretion, and gain or loss of channel fu...