Chemically defined serum-free media for rat hepatocytes have been useful in identifying EGFR ligands and HGF/MET signaling as direct mitogenic factors for rat hepatocytes. The absence of such media for mouse hepatocytes has prevented screening for discovery of such mitogens for mouse hepatocytes. We present results obtained by designing such a chemically defined medium for mouse hepatocytes and...

          Leishmaniasis is caused by an intracellular parasite . It endemic in Asia, Africa, the Americas, and Mediterranean region. Worldwide, 1.5 to 2 million new cases occur each year .The histological study of liver tissue white laboratory mice (Mus musculus) infected with L. major a showed The presence severe steatosis hepatocytes Hepatocyte deg...

The effect of antiviral therapy during Hepatitis C Virus (HCV) infection is the focus this study. HCV destroys healthy hepatocyte cells in human liver, causing cirrhosis and hepatocellular carcinoma. We introduce a cell-population model representing long-term dynamics response to drug therapies. proliferation existing can create system. Such models are based on susceptible hepatocytes, infected...

Porcine hepatocytes transplanted during acute liver failure might support metabolic functions until the diseased liver recovers its function. Here, we isolated high numbers of viable pig hepatocytes and evaluated hepatocyte functionality after encapsulation. We further investigated whether coculture and coencapsulation of hepatocytes with human multipotent mesenchymal stromal cells (MSC) are be...

BACKGROUND AND AIM The interaction between hepatitis C virus (HCV) and innate antiviral defense systems in primary human hepatocytes is not well understood. The objective of this study is to examine how primary human hepatocytes response to HCV infection. METHODS An infectious HCV isolate JFH1 was used to infect isolated primary human hepatocytes. HCV RNA or NS5A protein in the cells was dete...

As an intracellular pathogen, the reproduction of hepatitis B virus (HBV) depends on occupancy host metabolism machinery. Here we test a hypothesis if HBV may govern biosynthesis to achieve productive reproduction. To this hypothesis, set up affinity purification screen for factors that interact with large viral surface antigens (LHBS). This identified pyruvate kinase isoform M2 (PKM2), key reg...

background: in recent years we have successfully adopted an in vitro hepatogenic differentiation of mesenchymal stem cells (mscs). in this protocol the biologically active hepatocyte-like cells were differentiated from the stem cells isolated from either bone marrow or umbilical cord blood (ucb) samples. the aim of the present study was to compare the efficiency of the hepatogenic differentiati...

The liver is an important site of host-microbe interaction. Although hepatocytes have been reported to be responsive to lipopolysaccharide (LPS), the global gene expression changes by LPS and mechanism(s) by which LPS stimulates cultured hepatocytes remain uncertain. Cultures of primary mouse hepatocytes were incubated with LPS to assess its effects on the global gene expression, hepatic transc...

Hepatocytes isolated from rats fed on a chow diet or a low-protein (8% ) diet were used to study the effects of various factors on flux through the branched-chain 2-oxo acid dehydrogenase complex. The activity of this complex was also determined in cell-free extracts ofthe hepatocytes. Hepatocytes isolated from chow-fed rats had greater flux rates (decarboxylation rates of3-methyl-2-oxobutanoat...

introduction: chemokines are low molecular weight proteins (8-17kda) with the main role of immune cells recruitment to injured tissues. ip-10/mob-1 is a cxc chemokine and different cell systems in response to external stimulation produce this chemokine. various signaling pathways are used by cell and tissue systems to regulate production of proteins e.g. chemokines. therefore we have investigat...