Isothermal DNA amplification is an alternative to PCR-based amplification for point-of-care diagnosis. Since the early 1990s, the approach has been refined into a simple, rapid and cost-effective tool by means of several distinct strategies. Input signals have been diversified from DNA to RNA, protein or small organic molecules by translating these signals into input DNA before amplification, t...

In this article, failure and fracture mechanisms in an aluminum alloy (which has been used in diesel internal combustion engines), with and without ceramic thermal barrier coatings, have been investigated under isothermal and non-isothermal fatigue loadings. In this research, the base material is an aluminum-silicon-magnesium alloy and the thermal barrier coating includes a metallic bond coat l...

effects of ?-cyclodextrin, ?cd, on refolding of lysozyme was investigated at ph 12 employing isothermal titration calorimetry (itc) at 300k in 30mm tris buffer solution. ?cd was employed as an anti-aggregation agent and the heats obtained for lysozyme+?cd interactions are reported and analyzed in terms of the extended solvation model. it was indicated that there are two sets of identical and no...

poly(ethylene adipte) and poly(ethylene adipate)/silica nanocomposite (pead/sio2) containing 3 wt. % sio2  were prepared by an in situ method. the examinations on the non-isothermal crystallization kinetic behavior have been conducted by means of differential scanning calorimeter (dsc). the avrami, ozawa, and combined avrami and ozawa equations were applied to describe the crystallization kinet...

We study the structure of high-speed zero-pressure-gradient turbulent boundary layers up to friction Reynolds number $Re_{\tau } \approx 2000$ using direct numerical simulation Navier–Stokes equations. Both supersonic and hypersonic conditions with nominal free-stream Mach numbers $M_{\infty }=2$ , }=5.86$ heat transfer at wall are considered. The present simulations extend database currently a...

Dimerization process is essential for producing copolymers. The features of dimerization process like thermal-oxidative degradation should be well known to reach maximum efficiency and a superior reactor design. Also, the degradation mechanism of biodegradable polymers is important during sterilization processes. Thermal-oxidative degradation of PGA, PLLA, and their binary copolymers was invest...

We described an electrochemical method to monitor in real-time the isothermal helicase-dependent amplification of nucleic acids. The principle of detection is simple and well-adapted to the development of portable, easy-to-use and inexpensive nucleic acids detection technologies. It consists of monitoring a decrease in the electrochemical current response of a reporter DNA intercalating redox p...

This review covers both the history and present state of the kinetics of thermally stimulated reactions in solids. The traditional methodology of kinetic analysis, which is based on ® tting data to reaction models, dates back to the very ® rst isothermal studies. The model ® tting approach suŒers from an inability to determine the reaction model uniquely, and this does not allow reliable mechan...