The biosynthesis of pyridine dinucleotide transhydrogenase has been studied in isolated rat hepatocytes and in a rabbit reticulocyte-lysate translation system supplemented with either intact isolated rat liver mitochondria or the soluble matrix fraction from isolated mitochondria. In intact hepatocytes, the transhydrogenase precursor was short-lived in the cytosol and was efficiently imported i...

Abstract Background: Oral squamous cell carcinoma (OSCC) is the most frequent type of oral cancer. The tumor microenvironment OSCC induces an alteration T lymphocyte, promoting exhausted phenotype. main organelle metabolism mitochondrion and in recent years it has been indicated that several cells have capacity to transfer mitochondria, including cancer cells. However, not evaluated whether mit...

A comparison was conducted of current methods for estimation of the activity states (proportion of enzyme in active, dephosphorylated, form) of hepatic branched-chain 2-oxo acid dehydrogenase. Practically all of the enzyme was active in freeze-clamped liver obtained from chow-fed and 48 h-starved rats, regardless of the presence of fluoride in the extraction and assay media to inhibit phosphata...

Aberrant mitochondrial energy transfer underlies prevalent chronic health conditions, including cancer, cardiovascular, and neurodegenerative diseases. Mitochondrial transplantation represents an innovative strategy aimed at restoring favorable metabolic phenotypes in cells with dysfunctional energy metabolism. While promising, significant barriers to in vivo translation of this approach abound...

1. Prior treatment of rats with certain steroid hormones affects the binding of calcium to subsequently isolated liver mitochondria in vifro. Mitochondria isolated from animals treated with cortisol bind less calcium than do mitochondria from control animals under conditions in which binding is supported either by adenosine triphosphate alone or primarily by a respiratory substrate. Treatment w...

mitochondrial space was not completely reduced. This indicates some mitochondrial oxidative activity when alveolar Po, was in the 0.5-1.0 mrn Hg range. These studies have demonstrated substrate oxidation and respiratory control with isolated lung mitochondria. Measurements of surface fluorescence and tissue redox couples show intracellular reduction of pyridine nucleotides in response to oxidat...

Besides skeletal muscle dysfunction, Duchenne muscular dystrophy (DMD) exhibits a progressive cardiomyopathy characterized by an impaired calcium (Ca 2+ ) homeostasis and mitochondrial dysfunction. Here we aimed to determine whether sarco-endoplasmic reticulum (SR/ER)–mitochondria interactions function were in dystrophic heart at the early stage of pathology. For this purpose, ventricular cardi...

Multiple Sclerosis (MS) is a neurodegenerative and autoimmune disease that it’s molecular etiology and factors involving in its progression remains unknown. In this study for evaluation effect of mercuric on progression of MS we investigated the additive effect of mercuric sulfide on the brain mitochondrial dysfunction in experimental autoimmune encephalomyelitis (EAE) model of MS in C57BL/6 mi...

Despite recent improvements in treatment, ovarian cancer is still the leading cause of death from gynaecological malignancies. Today, marine mollusks are considered as natural source of new biologically and pharmacologically active compounds by scientists and the pharmaceutical industries. The aim of this study is to investigate the selective apoptotic effects of Turbo coronatus crude extract f...

The formation of polypeptides within the mitochondrion has been documented by a large body of evidence. The ability of isolated mitochondria to incorporate amino acids into membrane protein is an expression of this cellular capacity (for reviews see [ l-61 ). However, until now it has not been demonstrated that isolated mitochondria are able to synthesize definite polypeptides which are compone...