A sensitive, fast, and stability-indicating isocratic reverse-phase ultra-performance liquid chromatography method was developed and validated for quantitative simultaneous determination of sodium methylparaben, sodium propylparaben and ketorolac tromethamine in topical dosage forms. Separation of all peaks was achieved by using acquity ethylene bridged hybrid C18 (50×2.1 mm, 1.7 μ) as stationa...

Levocetirizine hydrochloride is an orally active, non-sedative antihistamine used in the symptomatic relief of seasonal and perennial allergic rhinitis. The main objective of the proposed study is to develop and validate a dissolution method of Levocetirizine hydrochloride dosage form by reverse phase HPLC isocratic method. The method is optimized by various filteration methods such as centrifu...

An isocratic, rapid and reproducible high performance liquid chromatographic (HPLC) method for quantitative determination of chlorphenoxamine hydrochloride (CPHX), caffeine (CAFF) and 8-chlorotheophylline (CTHE) has been adopted. The optimal conditions for separation and quantitative determination of these compounds were a normal phase mode chromatography and isocratic elution at ambient temper...

A deoxycytidine analog, gemcitabine (dFdC), is effective for treating solid tumors and hematological malignancies. After being transported into cancer cells, dFdC is phosphorylated to dFdC triphosphate (dFdCTP), which is subsequently incorporated into the DNA strand, thereby inhibiting DNA synthesis. Intracellular dFdCTP is the critical determinant for dFdC cytotoxicity, so therapeutic drug mon...

The isolation of single homologs polyethylene glycol by preparative reversed-phase chromatography is investigated. A thermodynamic model developed accurately previously describes the retention times individual as function their size, temperature, and mobile phase composition under linear, diluted conditions. extended to predict limiting for linear gradient operation in applications. Isocratic g...