The secondary structure of human brain cytosolic and mitochondrial 'malic' enzymes purified to homogeneity has been investigated by Fourier-transform IR spectroscopy. The absorbance IR spectra of these two isoenzymes were slightly different, but calculated secondary-structure compositions were essentially similar (38% alpha-helix, 38-39% beta-sheet, 14% beta-turn and 9-10% random structure). Th...

The number of biological/biomedical applications that require AMS to achieve their goals is increasing, and so is the need for a better understanding of the physical, morphological, and structural traits of high quality of AMS targets. The metrics of quality included color, hardness/texture, and appearance (photo and SEM), along with FT-IR, Raman, and powder X-ray diffraction spectra that corre...

Far-infrared absorption spectra of small neutral and cationic niobium clusters containing 5 to 9 Nb atoms have been obtained by multiple photon dissociation spectroscopy of their argon complexes. The experimental far-IR spectra are recorded in the 85–600 cm−1 region and cover the range of the structure-specific vibrational fundamentals, i.e., the finger-print range, for these metal clusters. Th...

We have obtained room-temperature transient infrared difference spectra of the M412 photoproduct of bacteriorhodopsin (bR) by using a "rapid-sweep" Fourier-transform infrared (FT-IR) technique that permits the collection of an entire spectrum (extending from 1000 to 2000 cm-1 with 8-cm-1 resolution) in 5 ms. These spectra exhibit less than 10(-4) absorbance unit of noise, even utilizing wet sam...

We report the application of target factor analysis (TFA) to the identification of trace analytes in open-path Fourier transform infrared (OP/FT-IR) spectra. Results showed that for components such as methane and ammonia, for which the path-integrated concentration was greater than approximately 100 ppm.m, TFA yielded results that closely match corresponding reference spectra. Furthermore, it w...

The prostate gland is conventionally divided into zones or regions. This morphology is of clinical significance as prostate cancer (CaP) occurs mainly in the peripheral zone (PZ). We obtained tissue sets consisting of paraffin-embedded blocks of cancer-free transition zone (TZ) and PZ and adjacent CaP from patients (n = 6) who had undergone radical retropubic prostatectomy; a seventh tissue set...

Polished plane-parallel sections (1 mm in thickness) of fused mullite (2Al 2 O 3 · 1SiO 2) were exposed to a slowly flowing (≈ 100 ml/min) water vapor containing atmosphere (H 2 O/O 2 = 20/80) at a temperature of 1670 °C for 1.5, 3, 6 and 12h, respectively. The water vapor experiments on fused mullite were carried out together with experiments on mullite single crystals reported earlier [1]. Al...

Protonated polycyclic aromatic hydrocarbons (HPAHs) have been reported to have infrared (IR) bands at wavenumbers near those of unidentified infrared (UIR) emission bands from interstellar objects. We produced 1-C16H11 + and 1-C16H11 upon electron bombardment during matrix deposition of p-H2 containing pyrene (C16H10) in a small proportion. Intensities of absorption features of 1-C16H11 + decre...

We report the results of the Cornell Mid-IR Asteroid Spectroscopy (MIDAS) survey, a program of ground-based observations designed to characterize the 8–13 μm spectral properties of a statistically significant sample of asteroids from a wide variety of visible to near-IR spectral classes. MIDAS is conducted at Palomar Observatory using the Spectrocam-10 (SC-10) spectrograph on the 200-in Hale te...

The vibrational spectroscopy and relaxation of an anharmonic oscillator coupled to a harmonic bath are examined to assess the applicability of the time correlation function (TCF), the response function, and the semiclassical frequency modulation (SFM) model to the calculation of infrared (IR) spectra. These three approaches are often used in connection with the molecular dynamics simulations bu...