Currently available induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) do not ideally model cellular mechanisms of human arrhythmic disease due to lack of a mature action potential (AP) phenotype. In this study, we create and characterize iPS-CMs with an electrically mature AP induced by potassium inward rectifier (IK1) enhancement. The advantages of IK1-enhanced iPS-CMs include the...

Two studies were conducted to evaluate the effects of diet on phosphorus (P) digestion in dairy cattle. The objective of the first study was to evaluate the effects of forage and non-fiber carbohydrate (NFC) content on total P (TP) and inositol phosphatesP (IPs-P) digestion. Samples of feed, duodenal digesta and feces from a previously conducted study were analyzed for TP and IPs-P. In this stu...

Induced pluripotent stem (iPS) cells can be established from somatic cells. However, there is currently no established strategy to generate corneal epithelial cells from iPS cells. In this study, we investigated whether corneal epithelial cells could be differentiated from iPS cells. We tested 2 distinct sources: human adult dermal fibroblast (HDF)-derived iPS cells (253G1) and human adult corn...

Induced pluripotent stem (iPS) cells provide powerful tools for studying disease mechanisms and developing therapies for diseases. The 8p11 myeloproliferative syndrome (EMS) is an aggressive chronic myeloproliferative disorder (MPD) that is caused by constitutive activation of fibroblast growth factor receptor 1. EMS is rare and, consequently, effective treatment for this disease has not been e...

BACKGROUND AND PURPOSE The inferior petrosal sinus (IPS) is the main transvenous access route used to examine or treat lesions involving the cavernous sinus. To carry out these procedures successfully, one must have a detailed knowledge of the anatomy of the venous system around the junction of the IPS and the internal jugular vein (IJV). MATERIALS AND METHODS Eighty-three sides in 63 patient...

The formation of cytoplasmic stress granules and the innate immune response are two distinct cellular stress responses. Our study investigated the involvement of four innate immune proteins - retinoic-acid-inducible gene I (RIG-I, also known as DDX58), melanoma differentiation-associated gene 5 (MDA5, also known as IFIH1), IFN-β promoter stimulator (IPS-1, also known as MAVS) and protein kinase...

BACKGROUND: Fidelity scales are used to monitor adherence to evidence-based practices. The underlying assumption is that high fidelity predicts better outcomes. The IPS-25 is a fidelity scale measuring adherence to the Individual Placement and Support (IPS) model. A previous study found a significant association between the IPS-25 and competitive employment outcome. The current study sought to ...

PURPOSE Retinal pigmented epithelium derived from human induced pluripotent stem (iPS) cells (iPS-RPE) may be a source of cells for transplantation. For this reason, it is essential to determine the functional competence of iPS-RPE. One key role of the RPE is uptake and processing of retinoids via the visual cycle. The purpose of this study is to investigate the expression of visual cycle prote...