A new rapid iodination reagent, N1,N3,N5-tris[(2,4,6-trimethylpyridine)iodo(I)]-2,4,6-triphenyl-s-triazine trihexafluorophosphate, was synthesized in a modification of the established synthesis 2,4,6-triiodo-3,5-dimethylphenol presence bis(2,4,6-trimethylpyridine)iodo(I) hexafluorophosphate and used for precise post-modification mono- trisubstituted phenyl compounds. We performed triple iodinat...

The results of metabolic labeling studies and enzymatic treatments followed by analysis on polyacrylamide gels show that the external proteins of hamster fibroblast cell lines, which have been identified by lactoperoxidase-catalyzed iodination, do not contain sulphated mucopolysaccharides or hyaluronic acid and are probably unrelated to collagen. Several of the iodinated species comigrate with ...

Measurements of neck count-rates for personnel who regularly iodinated proteins with 125I showed that, generally, count rates were higher after iodination than before. The major pathway for the intake of 125I during iodination was through skin that was exposed to the free-iodine atmosphere of the fume hood. Neck count-rates were measured in 34 other subjects who were working with 125I. The rate...

Utilizing cell preparations that contained greater than 90% eosinophils and sodium azide to inhibit the lysosomal enzyme peroxidase, studies of eosinophil bactericidal activity were undertaken. Eosinophils did not kill bacteria as well as neutrophils, principally because of a reduced phagocytic capacity. In contrast to neutrophils, eosinophils had high resting iodination activity but failed to ...

The kinetics of iodination at the 3-position of 1.3-dirnethyland [3-2HI]-1 ,3-dimethyl-indolin-2-one are reported for buffered mildly alkaline 50% (w /w) aqueous methanol solutions at 25". Unlike the iodination of many ketones these reactions (even in the presence of excess of substrate and with large amounts of added iodide ion) do not follow a zero-order rate expression, but show behaviour in...

Children with protein calorie malnutrition have impaired immune functions. Immunoglobulin levels are low in children with severe protein deprivation, but are not affected in moderate or borderline protein malnutrition. Specific antibody response is either normal or reduced depending on the antigen used. Involution of the thymus dependent lymphatic system and severe impairment of the cell-mediat...

The reactivities of the tyrosyl residues of calf and rabbit cytochrome bs have been examined by acylation, iodination, and titration of the proteins. These experiments distinguish residues 6 and 7 as the most exposed and reactive residues, which ionize normally and can be acetylated or iodinated to form the monoiodo or diiodo derivatives. Tyrosine 30, which is common to both cytochrome preparat...

Conditions were established where the thallium-catalyzed iodination of random coil DNA proceeded 100-200 times faster than for native DNA. This reaction was explored as a probe for localized regions of disrupted base pairs in duplex DNA. A heteroduplex was constructed between DNA fragments produced by Hind II + III cleavage of phi80 plac DNA and phi80 plac DNA containing the Ll deletion (73 nuc...