Tilapia (Oreochromis niloticus) is the most cultivated and consumed freshwater fish in Brazil. The present study aimed to evaluate microbiological quality of ice fresh tilapia samples commercialized Federal District. were tested for counts mesophilic psychrotrophic bacteria, determination total thermotolerant coliforms, Staphylococcus aureus presence Salmonella. Ice analyzed coliforms Escherich...

the objective of the current study was to evaluate the reproducibility of a reverse transcriptase pcr (rt-pcr)-based technique to differentiate viable and dead salmonella cells in raw and sterilized milk. the microorganism was initially inoculated into the milk samples followed by incubating at 37°c for 4 h prior to inactivation by heat at 80°c for 10 min. the treated and non-treated samples we...

A total of 480 samples, comprising 429 faecal samples from healthy adult birds and 51 tissue samples from dead birds, were collected from four government poultry farms in the Kashmir valley from September 2007 to April 2008. In all, 33 Salmonella isolates were obtained. Of these, 28 (84.85%) isolates were Salmonella Gallinarum, 3 (9.09%) were Salmonella Enteritidis and the remaining 2 (6.06%) w...

The intestinal mucosa forms the first line of defense against infections mediated by enteric pathogens such as salmonellae. Here we exploited intestinal "organoids" (iHOs) generated from human induced pluripotent stem cells (hIPSCs) to explore the interaction of Salmonella enterica serovar Typhimurium with iHOs. Imaging and RNA sequencing were used to analyze these interactions, and clear chang...

AIM This work was conducted to study the phenotypic and genotypic characterization of locally isolated Salmonella strains (Salmonella Pullorum, Salmonella Enteritidis, and Salmonella Typhimurium) from poultry used in the preparation of Salmonella antigens in Egypt. MATERIALS AND METHODS The phenotypic characterization of Salmonella strains was done using standard microbiological, biochemical,...

An essential component of type III secretion systems (TTSS) is a supramolecular structure termed the needle complex. In Salmonella enterica, at least four proteins make up this structure: InvG, PrgH, PrgK, and PrgI. Another protein, PrgJ, is thought to play a role in the assembly of this structure, but its function is poorly understood. We have analyzed the expression and localization of PrgJ a...

By sequencing part of the wsp gene of a series of clones, we detected an unusually high diversity of nine Wolbachia strains in queens of three species of leafcutter ants. Up to four strains co-occurred in a single ant. Most strains occurred in two clusters (InvA and InvB), but the social parasite Acromyrmex insinuator hosted two additional infections. The multiple Wolbachia strains may influenc...