Background: in vitro maturation of human germinal oocytes is a promising technique assisted reproductive technologies, different medium has been introducing last two decays for this purpose but they are very expensive and gained by order with qualification Objective: the aim study to evaluate efficacy fertilization used vesicles during ICSI cycles. Patients methods: 42 vesicle stage involved (I...

BACKGROUND Intracytoplasmic sperm injection (ICSI) is an advanced technique employed in assisted reproductive clinics for treatment of infertile couples. The reproductive endocrinologists try their level best to identify factors that enhance success rate after ICSI. OBJECTIVE To compare estradiol progesterone ratio on ovulation induction day amongst pregnancy outcome groups following ICSI. ...

OBJECTIVE To observe effect of peak estradiol (E2) levels on outcome after intra cytoplasmic sperm injection (ICSI). METHODS Quasi experimental design conducted in "Islamabad Clinic Serving Infertile Couples" from June 2010 till August 2011. Down regulation with mid luteal suppression of 564 females aged 18-41 years was done with gonadotrophin releasing hormone agonist followed by controlled ...

The objective of this study was to determine the effects of method and clinician on stallion sperm morphology evaluation. Five clinicians evaluated 60 semen samples using wet-mount preparations with phase-contrast, eosin/nigrosin-stained semen smears, and Papanicolaou-stained semen smears. There were significant differences among methods for all sperm morphology categories and most intra-class ...

the purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (icsi) and piezo-assisted sperm injection after pretreatment with calcium ionophore (cai) on the mouse embryo development. methods: in this study, the conventional icsi and piezo-icsi procedures were used. the efficacy of the methods was examined after mouse matured oocytes were fertilized with or witho...

Objective: Our study was undertaken to find if any correlation exists between levels of Anti-Mullerian (AMH), follicle stimulating hormone (FSH) and estradiol (E2), with the ovarian reserve. Methods: A Randomized, prospective study was conducted at Infertility Clinic,Department of Obstetrics & Gynecology, Mahatma Gandhi Medical College and Hospital,Jaipur on 105 patients aged 25-40 years underg...

Abstract Study question How does sperm phospholipase C zeta (PLCζ) affect fertilization in conventional vitro (cIVF)? Summary answer Absence of PLCζ causes polyspermy, and a decline the proportion expressing is correlated with low rate (FR) after cIVF. What known already key sperm-borne factor that triggers Ca2+ oscillations subsequent oocyte activation following gamete fusion. Mutations PLCZ1,...

BACKGROUND In couples presenting with retrograde ejaculation refractory to medical treatment, the first choice of fertility treatment should be Assisted Reproductive Techniques using rapidly purified spermatozoa retrieved from post-ejaculatory urine. The Hotchkiss technique and modified variants are simple and efficient for retrieving sperm from the bladder. We developed a new protocol, includi...