Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. Pseudomonas sp. MCCMB3 an alpha galactosidase producer was isolated from the rock soil of Kulasekaram, Kanyakumari district, South India. Totally 10 strains were isolated, when compared with other strains, predominant enzyme activity has been observed in...

hairy root systems are formed by transforming plant tissues with the “natural genetic engineer” agrobacterium rhizogenes. in most plants such as cichorium intybus l., hairy root cultures have proven to be an efficient system for secondary metabolites production. the effect of zinc (znso4), a heavy metal, was investigated at different concentrations (0, 1, 5 and 10 mm) on some secondary metaboli...

asparaginase and amylase are widely used enzymes in various industries, which can be produced by endophytic fungi. in this study, the ability of producing these two enzymes in endophytic fungi isolated from six species of thymus has been reported for the first time in the world. among 89 isolates of the test, 34 isolates produced asparaginase among which m24 (fusarium subglutinans) displayed th...

Enzymes are capable of unique and selective transformations that can enable efficient chemical production. While many industrial processes have been developed using free enzymes in aqueous solutions, immobilizing on a solid support offer considerable advantages, including improved reaction efficiency, enzyme stability, the ability to perform reactions non-aqueous media, simplified separation pr...

Lysolecithin is preferentially used in food technology, in animal feed products, and in cosmetics and pharmaceutical preparations as a surfactant.i} Industrially, lysolecithin s produced by enzymatic hydrolysis of Iecithin, The most eommon enzyme used is pancreatin prepared from porcine pancreas. However, the use of pancreatin has certain disadvantages, such as its limited supply, its high cost...

A fungal strain of Aspergillus oryzae was used for the production of alpha amylase by solid state fermentation from agro-industrial waste. Enzyme production was growth associated and maximum activity (8.23 U/ml) were obtained after 120h when incubated at 30°C on wheat bran with initial moisture content 60%; initial medium pH = 5 . Enzyme activity increased when the solid medium was supplemented...

This study presents production of α-amylase by Aspergillus oryzae in solid-state fermentation using 14 agro-industrial wastes as substrate. Enzyme production was growth associated and maximum titers (15095 U/gds) were obtained after 72 h when incubated at 30°C on wheat bran (initial moisture content, 60%; initial medium pH, 5). Enzyme titers increased significantly when the solid medium was sup...

This paper reports the covalent immobilization of penicillin G acylase from E. coli on sepabeads EC-EP, an epoxy-activated polymethacrylic carrier, and describes kinetic properties of the immobilized enzyme. The selected enzyme belongs to a class of biocatalysts whose industrial interest is due to their versatility to mediate hydrolysis of penicillins and semi-synthetic β−lactam antibiotics syn...