Analysis of neutral and acidic ethyl acetate extracts from culture medium of Azospirillum brasilense 703Ebc by high-performance liquid chromatography (HPLC) and combined gas chromatography-mass spectrometry demonstrated the presence of indole-3-acetic acid (IAA), indole-3-ethanol, indole-3-methanol, and indole-3-lactic acid. IAA in media of 20 strains of A. brasilense and Azospirillum lipoferum...

Indole-3-acetic acid (plant auxin) has low toxicity but dramatically enhances the killing of mammalian cells on illuminating phenothiazinium dyes with red light. Suitable dyes include toluidine blue, used in cancer diagnosis because of localization in tumors, and methylene blue, used in experimental photodynamic therapy of cancer. The photosensitized oxidation of indole acetic acid forms a free...

Nodal explants of Phyla nodiflora (L.) Greene, were cultured on Murashige and Skoog (MS) medium supplemented with different combination and concentration of cytokinins and auxins for multiple shoots regeneration. The maximum numbers of shoots were found in MS medium supplemented with2.5 mgl-16-benzylaminopurine (BA) and 0.5 mgl-1 kinetin (KN). Elongated shoots were transferred to rooting ...

BACKGROUND Chlorophytum borivilianum is an industrially valued medicinal crop. Propagation through seeds is not feasible because of low germination percentage and long dormancy period. Therefore, callus culture and plant regeneration can be an alternative to improve this crop production. Also, callus can serve as an alternative source of bioactive compounds. OBJECTIVE To evaluate the effect o...

The use of Escherichia coli as a model organism for studies on the biochemistry of the action of indole-3-acetic acid has revealed that two products of the photooxidation of this plant hormone, 3.hydroxymethyloxindole and 3-methyleneoxindole, are potent inhibitors of the growth of both microorganisms and higher plants (I). The latter compound was first identified by Hinman, Bauman, and Lang (2)...

Low nitrogen stress severely impedes crop growth and productivity. There has been substantial research on root adaptation to low conditions in many plant species. However, the mechanism underlying morphological response of strawberry (Fragaria × ananassa Duch.) low-NO3− or low-NH4+ remains poorly understood. Strawberry plants were hydroponically cultivated under 1 mM NO3−, NH4+, control (15 NO3...

Drugs-of-abuse screening is becoming more prevalent, and a major technical and legal concern is that individuals may be mistakenly identified as drug usersbecause of a false-positive test result. Marijuana use can be detectedby screening a urine specimen with the Abbott (North Chicago, IL) TDx cannabinoid assay, which gives a positive reaction with several delta-9-tetrahydrocannabinol (THC) der...

Indole-3-acetic acid (IAA) is the most important member of the group of phytohormones known as auxins, which regulate many aspects of plant growth and development. The concentration of IAA in cells and tissues is controlled by various mechanisms, including de novo biosynthesis, synthesis and hydrolysis of IAA conjugates, conversion of IAA to indole-3-butyric acid (IBA), and oxidative degradatio...

The influence of growth regulators on the root induction of the Musa genus plants cultivated within in vitro conditions has been compared. The plant material used was the ‘Cavendish’ cultivar. Different concentratios of growth regulators (naphthaleneacetic acid, indole-3-acetic acid, 6-benzylaminopurine, 2.,4-dichlorophenoxyacetic acid) has been used and as control ones MS and half concentrated...

Release of divalent ions from membrane pellets of soybean hypocotyls was promoted by the natural auxin, indole-3-acetic acid, and the synthetic auxin, 2,4-dichlorophenoxyacetic acid. The calcium release occurred at auxin concentrations as low as 1 nanomolar, and maximum release was observed at 1 micromolar. Hormone concentrations greater than 1 micromolar showed reduced effectiveness in releasi...