The cost of the device, as shown in Figure 1, was estimated using low-volume pricing for all materials and reagents (Table S1). Once scaled-up for high-volume manufacturing, bulk discounts would further decrease the total cost. The transition from 25 µL LAMP reactions in tubes, to 10 µL reactions run in FTA paper, required optimization of the reaction conditions. Here, we investigated two diffe...

The growth characteristics of L. chagasi (MHOM/BR/79/LI01) and L. braziliensis (MHOM/BR/72/1670), the causative agents of visceral and muco-cutaneous leishmaniases, respectively, were compared. Inoculum size clearly influences the growth course of both Leishmania species, whatever the culture medium used (serum-supplemented media: GLSH or RPMI, and a chemically defined medium: LITR9). Cultures ...

We outline a new multiple trace theory of temporal preparation (MTP), which accounts for behavior in reaction time (RT) tasks in which the participant is presented with a warning stimulus (S1) followed by a target stimulus (S2) that requires a speeded response. The theory assumes that during the foreperiod (FP; the S1-S2 interval) inhibition is applied to prevent premature response, while a wav...

Duas novas flavanonas naturais, 5-hidroxi-6,7-(2”,2”-dimetilcromeno)flavanone (1) e 5-metoxi3-hidroxi-6,7-(2”,2”-dimetilcromeno)flavanone (2), foram isoladas dos extratos das raízes de L. yucatanensis e L. xuul, respectivamente. Adicionalmente, quatro novos metabólitos, os flavanóides glicosídicos quercetina-3-ramnoglucosídeo e caempferol-3-ramnoglucosídeo, juntamente com 4hidroxi-N-metil-proli...

Most of the ocean is deep with majority its volume (> 80%) lying under a depth greater than 1000 m. Deep-ocean substrates input mainly supplied as organic matter (in particulate and/or dissolved forms) by physical and biological processes. Bioavailable carbon (DOC) consumed in surface water prokaryotes, while most DOC recalcitrant. Deep-sea prokaryotes are known to be adapted degrade complex su...

1. The catabolism of glycine was studied in isolated rat liver mitochondria by measuring release of 14CO2 from [1-14C]-glycine. Incubation of mitochondria in a medium containing 0.5 microM free Ca2+ resulted in an 8-fold increase in the rate of degradation of glycine. Intraperitoneal injection of glucagon (33 or 100 micrograms/100 g body wt.) 25 min before killing of rats also resulted in a 3-f...

in this study, the effectiveness of kiwi shell was investigated to reduce the mobility of lead and cadmium in clay soil in different intervals. for this purpose a clay soil sample was contaminated with lead and cadmium in distinct dishes with 10 and 600 ppm concentrations respectively and mixed with 5% kiwi shell. samples were placed in incubator, and then sampling of soil in incubator was perf...