Background: In this feasibility study, a mechanically loaded in-vitro tissue culture model of joint morphogenesis using the isolated lower extremity of the 8 day old chick embryo was developed to assess the effects of mechanical loading on joint morphogenesis. Methods: The developed in-vitro system allows controlled flexion and extension of the chick embryonic knee with a range of motion of ...

The effect of in ovo administration of antiadipocyte monoclonal antibodies (MAb) on growth and adipose tissue development in chickens was investigated. In Trial 1, 60 fertilized eggs from layer-type chickens were injected into allantoic circulation with different amounts of MAb against chicken adipocyte plasma membranes (0, 0.05, 0.1, 0.2, and 0.4 mg) on Day 15 of embryogenesis. In Trial 2, 180...

The capacity to image a growing embryo while simultaneously studying the developmental function of specific molecules provides invaluable information on embryogenesis. However, until recently, this approach was accomplished with difficulty both because of the advanced technology needed and because an easy method of minimizing damage to the embryo was unavailable. Here, we present a novel way of...

In ovo feeding (IOF), injecting dietary components into the amnion about 1 d prior to internal pipping, may enhance growth by altering glycogen status. This hypothesis was evaluated with 5 IOF solutions containing protein, β-hydroxy-β-methylbutyrate (HMB), and carbohydrate. Four IOF treatments were arranged as a factorial of 2 levels of egg white protein (EWP; 0 and 18%) and 2 levels of HMB (0 ...