In her article "Science, Sexuality, and the Novels of Huxley and Houellebecq," Angela C. Holzer begins with an introduction to recent discourse about contemporary culture by Francis Fukuyama, notably in his book Our Posthuman Future (2001). Next, Holzer introduces twentiethcentury literary representations of genetic engineering. Focusing on Huxley's Brave New World (1932) and on Houellebecq's L...

RECOMBINANT POETICS Interaction with different forms of generative production enables one to dynamically explore emergent meaning. New forms of computer-based art can make it possible for participants to actively become engaged in aspects of the production of the work. Each “user” of a specifically authored computer-mediated system may have a quite different experience emerging through interact...

Poet (Prototype Object Extension for Tcl) extends the Tcl language with objects featuring dynamic, prototype-based inheritance, persistence, and one-way constraints between object attributes. Poet includes wrappers around the Tk and BWidget widgets that are automatically generated using introspection. This paper also describes Poetics (Poet Integrated Construction Set), a sub-project within Poe...

This article examines how documentary poetics—particularly as employed by Muriel Rukeyser—use a montage of images to form visual landscape. landscape is wielded effectively politicians during the Whistle Stop Tour electioneering. In Rukeyser’s “Campaign”, one section in her long-form poetic biography Wendell Willkie, entitled One Life, she describes journey 1940 Republican presidential candidat...

BACKGROUND A versatile strategy for protein-surface coupling in biochips exploits the affinity for polyhistidine of the nitrilotriacetic acid (NTA) group loaded with Ni(II). Methods based on optical reflectivity measurements such as spectroscopic ellipsometry (SE) allow for label-free, non-invasive monitoring of molecule adsorption/desorption at surfaces. RESULTS This paper describes a SE stu...

Here we describe a generic procedure for the expression and purification of milligram quantities of functional recombinant eukaryotic integral membrane proteins, exemplified by hexahistidine-tagged bovine rhodopsin. These quantities were obtained with the recombinant baculovirus/Sf9 insect cell-based expression system in large-scale bioreactor cultures with the use of a serum-free and protein-f...

The crystal structure of a mutant form of the single-chain fragment (scFv), derived from the monoclonal anti-His tag antibody 3D5, in complex with a hexahistidine peptide has been determined at 2.7 A resolution. The peptide binds to a deep pocket formed at the interface of the variable domains of the light and the heavy chain, mainly through hydrophobic interaction to aromatic residues and hydr...

The cDNA of the human GM2-activator protein was cloned into the expression vector pHX17. The plasmid encodes a fusion protein with a hexahistidine tail and a Factor Xa cleavage site at its N-terminus. The recombinant protein was purified from cell homogenates under denaturing conditions by metal-ion affinity chromatography in a single step and then was refolded. The hexahistidine tail could be ...