Immunoperoxidase positive HPP-producing cells have been examined ultrastructurally using the serial semithin-thin section technique. The features of the HPP cells were found to be identical with those of the previously described D1-cell. The D1-cells are scattered throughout the exocrine parenchyma and occur to a minor extent in the islets.

By using immunoperoxidase cytochemistry at the light and electron microscopic level, microtubles were visualized in a number of "normal" nontumorigenic and transformed tumorigenic cell lines. A well-defined cytoplasmic microtubule complex exists in both normal and transformed interphase cells. The distribution of this complex closely correlates with the cell shape and the degree of cell spreadi...

H]glucosamine has been used to label metabolically keratinocyte cell-surface glycoconjugates. The major labelled bands identified on sodium dodecyl sulphate/polyacrylamide gels had apparent molecular weights of greater than 250000, and 150000-80000. Most of these components were trypsin-sensitive, indicating that the label was protein-bound. Some of the labelled components were shown to be prot...

An immunoperoxidase technique for the optical microscopic detection of cellular immunoglobulin has been used to stain fixed smears of human neoplastic B lymphoid cells. Only four out of 28 cases of chronic lymphatic leukaemia (CLL) showed membrane labelling by this technique. In contrast, when 14 samples from other types of B lymphoproliferative disorder (including hairy cell leukaemia, non-Hod...

Tamm-Horsfall glycoprotein was isolated from hamster urine, and antiserum against it was produced in rabbits. IgG was isolated from the antiserum. Immunocytochemical methods were used to localize Tamm-Horsfall-like immunoreactivity in three substrains of baby-hamster kidney (BHK) cells. Indirect immunofluorescence techniques showed that, in two substrains (BHK-21/C13/2P and BHK-21/C13/3P), a pr...

Histopathologic (hematoxylin and eosin [HE]) and immunoperoxidase (streptavidin-biotin complex) methods were used for examination of formalin-fixed tissues of rabid raccoons from an enzootic area of Pennsylvania. Extensive morphologic lesions of rabies encephalitis were present in the cerebrum and the brain stem regions. Negri bodies were detected by both methods and were present in the brain (...

An immunoperoxidase method using a monoclonal antiserum that recognizes 8-hydroxydeoxyguanosine has been developed for detection and quantitation of oxidative damage in single cells. The method was initially applied to cultured cells treated with H2O2 or aflatoxin B1 and then to cryostat liver sections of rats treated with aflatoxin B1. To demonstrate that the method has sufficient sensitivity ...

The distribution of light and heavy immunoglobulin chains in chronic lymphocytic leukemia (CLL) cells has been investigated at the ultrastructural level using an immunoperoxidase technique. Light chains were localized in the lumens of the perinuclear space and rough endoplasmic reticulum, while staining for heavy chains was weak or negative and generally confined to the membranes of the rough e...

A reliable immunoperoxidase schedule for the cellular demonstration of tumour and normal cell products which can be completed in well under two hours is described. The preparations are as clear and readable as those stained by routine histological techniques and should form a valuable adjunct in research and diagnostic studies.