Intracellular life of Helicobacter pylori inside Candida yeast vacuole describes the establishment of H. pylori in yeast as a pre-adaptation to life in human epithelial cells. IgY-Hp conjugated with fluorescein isothiocyanate (FITC) has been previously used for identification and localization of H. pylori inside the yeast vacuole. Here we examined whether FITC-IgY-Hp internalization into yeast ...

Group A Rotaviruses are the most common cause of severe, dehydrating diarrhea in children worldwide. The aim of the present work was to evaluate protection against rotavirus (RV) diarrhea conferred by the prophylactic administration of specific IgY antibodies (Ab) to gnotobiotic piglets experimentally inoculated with virulent Wa G1P[8] human rotavirus (HRV). Chicken egg yolk IgY Ab generated fr...

The IgY-technology stand for the immunisation of chickens and the extraction of specific IgY antibodies (Ab) from egg yolk. Due to the non-invasive Ab isolation IgY-technology is internationally accepted as so-called alternative method. Despite of the aspect of animal welfare Ab production in chicken offers also outstanding economical advantages. i) purchase of chickens is much more cheep than ...

The reference method for immunoglobulin G (IgG) avidity determination includes reagent-consuming serum titration. Aiming at better IgG avidity diagnostics, we applied a logistic model for the reproduction of antibody titration curves. This method was tested with well-characterized serum panels for cytomegalovirus, Epstein-Barr virus, rubella virus, parvovirus B19, and Toxoplasma gondii. This ap...

The general structure of the egg yolk antibody, containing of two light and two heavy chains, is comparable to that of mammalian immunoglobulins. Oral administration of specific egg yolk antibody is an attractive approach against gastrointestinal infection in humans and animals. The use of IgY as antibioticalternative therapy have advantages: (1) The production of IgY is a non-invasive and inex...

Salmonella enteritidis (SE) colonizes the intestinal tract of poultry and causes food born illness in humans. Reduction of (SE) colonization in the intestinal tract of poultry reduces potential carcass contamination during slaughter. The purpose of this study was to investigate the effect of SE-specific yolk immunoglobulin (IgY) on prevention of SE colonization in orally infected broiler chicke...

OBJECTIVES As an alternative therapeutic approach for the prevention and treatment of bacterial infections with P. aeruginosa of cystic fibrosis (CF) patients, chicken yolk antibodies (IgY) could be used. The most significant advantage of IgY, in contrast to mammalian IgG, consists in the fact, that when bound to the antigen, they usually do not induce inflammatory reaction. In addition, the si...

Previously, the protective efficacy of antiAspergillus fumigatus IgY antibodies; prepared in egg laying hens, was reported in immunocompetent BALB/c mice. Since A. fumigatus infections are of main concern in immunocompromised patients, the aim of this study was to test the efficacy of the same product in Cyclosporine A treatedBALB/c mice. Heatkilled A. fumigatus was emulsified with both Freund’...

Porphyromonas gingivalis gingipains is suspected to be one of the most important causative agents of periodontitis. We postulated that the inhibition of gingipains may reduce the pathogenic nature of P. gingivalis. Anti-P. gingivalis egg yolk antibody (IgY-GP) was isolated from the yolks of hens immunized with purified gingipains. We applied IgY-GP gel subgingivally in periodontitis patients wh...

Clostridium difficile infection (CDI) are the leading cause of world-wide nosocomial acquired diarrhea. The current main clinical challenge in CDI is the elevated rate of infection recurrence that may reach up to 30% of the patients, which has been associated to the formation of dormant spores during the infection. We sought to characterize the effects of oral administration of specific anti-sp...