A study of the precipitability by the appropriate antisera of 34 different haptens, containing from one to six reactive groups, leads to the conclusion that the possibility of framework ("lattice") formation is neither necessary nor sufficient for specific precipitation, but that instead precipitation depends upon the reduction, by mutual neutralization of polar groups of antibody and antigen (...

Immune sera were produced in rabbits with living cells of an enteroinvasive O143 strain of Escherichia coli. To remove O and K antibodies, sera were absorbed with an avirulent derivative of the same strain. In the enzyme-linked immunosorbent assay, absorbed sera reacted specifically with only virulent Shigella strains and enteroinvasive E. coli strains of different geographical origin, regardle...

The immune response against the infecting group A streptococcus (GAS) extracellular products (EP) was determined in acute- and convalescent-phase sera from 75 patients with different clinical manifestations of GAS infection. All EP elicited a high proliferative response in human peripheral blood mononuclear cells. In patients with bacteremia, low neutralization in acute-phase sera was associate...

We have characterized a 120-Kd antigen that frequently reacts with serum antibodies from patients with immune thrombocytopenia or normal subjects. Immunoblots made after two-dimensional nonreduced/reduced sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) or two-dimensional isoelectric focusing/SDS-PAGE demonstrated that this 120-Kd protein has the same molecular weight under ...

One hundred and seven patients with colorectal carcinoma were examined for their immune response to autologous tumour. Patient leucocyte migration inhibition by tumour antigen, migration inhibition of normal subject's leucocytes by patient's serum, and the assessment of lymphocyte invasion of the tumour specimen were used to measure the immune response. Forty-one and 51% of patients were immune...

Sera of 22 individuals were examined for immunoglobulin M (IgM) and immunoglobulin G (IgG) antibody to autologous cultured tumor cells. Reactive native IgM was detected by immune adherence in four, and IgG by Protein A hemadsorption in five, sera. Direct and absorption testing of these reactive sera against a range of normal and neoplastic cells revealed one each with specificity for a highly r...

BACKGROUND Prior to detection of an antibody response toward influenza viruses using the hemagglutination inhibition assay (HAI), sera are routinely treated to inactivate innate inhibitors using both heat inactivation (56°C) and recombinant neuraminidase [receptor-destroying enzyme (RDE)]. OBJECTIVES We revisited the contributions of innate serum inhibitors toward interference with influenza ...

We measured the concentration of three immunoglobulin classes of rheumatoid factors (Ig-RFs), C3d binding IgG immune complex (C3d-IC), Clq binding IgG immune complex (Clq-IC) and C-reactive protein (CRP) in 74 samples of human sera with high levels of RF (24.0-2350.0 IU/mI). In sera with high levels of C3d-IC (> 15.0,ug/ml), there was a positive correlation between the levels of CRP and the IgG...

Immune sera were obtained from four rainbow trout that had survived natural infection by infectious haematopoietic necrosis virus (IHNV), and five monoclonal antibodies (MAbs) were prepared against a Korean isolate of IHNV, IHNV-PRT. These immune sera and MAbs were characterized in terms of IHNV-neutralizing properties and reactivity in Western blots with the viral proteins of IHNV-PRT. All fiv...