in endemic area the most challenging problem for brucellosis is to find a reliable diagnostic method. in this case-control study, we investigated the accuracy of elisa test for diagnosis of human brucellosis and determined the optimal cut-off value for elisa results in iran. the laboratory diagnosis of brucellosis was performed by blood isolation of brucella organism with a bactec 9240 system a...

Accurate determination of the concentrations of immunoglobulin G (IgG) antibody to tetanus toxoid is important in order to evaluate the immunogenicity of tetanus toxoid vaccines, determine immune competence in individual patients, and measure the prevalence of immunity in populations. The performance of three commercially available enzyme-linked immunosorbent assays (ELISAs) for IgG antibodies ...

Background: Dengue is an acute viral infection has emerged as a notable public health problem. Rapid immunochromatographic test and IgM/IgG ELISA been the mainstay of diagnosis. Combination NS1 antigen detection along with antibody increases diagnostic rates. Thrombocytopenia begins during febrile phase. Therefore, we tried to evaluate association platelet counts against (NS1 IgM/IgG) IgM in de...

The enzyme-linked immunosorbent assay (ELISA) was applied for immunological diagnosis of human tularemia, using lipopolysaccharide from Francisella tularensis as antigen. Sera collected from patients, healthy individuals, and vaccinated volunteers were investigated for antibodies against F. tularensis by ELISA and tube agglutination. In ELISA all sera were titrated with a polyspecific anti-immu...

Background: BK virus, a human polyomavirus, causes nephropathy and allograft loss in renal transplant recipients. Although it was discovered 1971, understanding of the humoral immune response to BKV is limited.
 Objective: To serological detection level estimation anti-BK-IgG renal-transplanted recipients healthy blood donors as control.
 Patients Methods: Serum samples were collected...

PURPOSE This study evaluated the prevalence of ocular toxocariasis (OT) in patients with uveitis of unknown etiology who visited a tertiary hospital in South Korea and assessed the success of serum anti-Toxocara immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) as a diagnostic test for OT. METHODS The records of consecutive patients with intraocular inflammation of unknown etio...

A dot enzyme-linked immunosorbent assay (dot ELISA) was evaluated and compared with a standard microplate ELISA (immunoglobulin G [IgG] ELISA) for the serological diagnosis of mucocutaneous leishmaniasis. The two assays were used to test 113 serum specimens from the following groups: normal individuals and patients with deep mycoses, toxoplasmosis, mucocutaneous leishmaniasis, visceral leishman...

OBJECTIVE Serology and (13)C-urea breath test have been widely used as noninvasive tests to detect Helicobacter pylori infection. However, easier collection of samples and lower costs are desirable for diagnosis of the individual patient or for use in epidemiologic studies. Our aim was to study the diagnostic accuracy of a recently developed urine-based enzyme-linked immunosorbent assay (ELISA)...

background and objectives: brucellosis is one of the most common zoonotic diseases in iran and human brucellosis is endemic in all parts of the country. because of the difficulty in the diagnosis of brucellosis, particularly in endemic areas, the use of new and feasible diagnostic tests seem to be of great importance for resolving the diagnostic obstacles. we evaluated the usefulness of a new s...