Recent evidence has highlighted the critical role of memory cells in maintaining lifelong food allergies, thereby identifying these as therapeutic targets. IgG+ B replenish pools IgE-secreting upon allergen exposure, which contract thereafter due to short lifespan tightly regulated IgE-expressing cells. Advances detection and highly dimensional analysis allergen-specific T from allergic patient...

Abstract The rising incidence of allergic disease is a public health challenge needing novel interventions. Mast cell activation by IgE an established feature allergy, but fundamental aspects IgE-induced signaling remain unclear. Prior data from our lab testing the effects fluoxetine on IgE-driven mast function indicated role for purinergic receptor P2X3. P2X3 ATP-activated cationic channel mos...

Several splice variants of IgE exist in human plasma, including a variant called IgE-tailpiece (IgE-tp) that differs from classical IgE by the replacement of two carboxy-terminal amino acids with eight novel residues that include an ultimate cysteine. To date, the role of the secreted IgE-tp isoform in human immunity is unknown. We show that levels of IgE-tp are raised in helminth-infected dono...

Background. Allergen-specific immunotherapy (SIT) is one of the important regimens for the treatment of allergic diseases. Predictive tests for the clinical response to SIT are limited. In this study we aimed to evaluate whether specific IgE/total IgE levels can predict clinical improvement in monosensitized patients to house dust mite treated with immunotherapy. Patients and Methods. We analyz...

Chronic rhinitis (CR) is commonly divided into allergic (AR) and nonallergic (NAR). AR triggered by the immunoglobulin E (IgE)-mediated response to allergens, whereas NAR characterized absence of sensitization. Previous studies have demonstrated presence local IgE in nasal mucosa patients suffering from typical symptoms but without a history atopy positive allergen challenge (NAC). This conditi...

As reported previously, and confirmed here in 26 donors, the serum IgE level (2.6-5,500 ng/ml) correlates well (rs = 0.95, P less than 0.001) with the in vivo number of IgE molecules/basophil (6,000-600,000). The total number of IgE receptors/basophil was monitored by incubating them with an IgE-rich serum (15 microgram/ml), quantitatively stripping IgE from the cells at pH 3.7, and measuring e...

Results In 16 patients (42.1%) with negative serum specific IgE tests for both fungi and HDM, total IgE ( 76.1 + 64.7 KU/L) was significantly lower than in 14(36.8%) patients with positive serum specific IgE tests for fungi (730.1 + 948.41 KU/L) and in 16 patients (42.1%). with positive serum specific IgE tests for HDM (783.5 + 854.1 KU/L), respectively. In patients with negative serum specific...

BACKGROUND Studies have demonstrated that IgE-binding cross-reactive epitopes between shrimp, cockroach, and house dust mite tropomyosins can account for the presence of detectable IgE to shrimp in patients with cockroach and dust mite allergies. OBJECTIVE We investigated the correlation between IgE-mediated sensitization to shrimp, cockroach, and dust mite in relation to allergen exposure in...

Release of pro-inflammatory mediators by mast cells is a key feature of allergic disease. The 'dogma' is that IgE molecules merely sensitise mast cells by binding FcεRI prior to cross-linking by multivalent allergen, receptor aggregation and mast cell activation. However, certain monoclonal IgE antibodies have been shown to elicit mast cell activation in an antigen-independent cytokinergic mann...