We report here the identification of a 24-kDa polypeptide in IBV-infected Vero cells by immunoprecipitation with a region-specific antiserum raised in rabbits against the IBV sequence encoded between nucleotides 10,928 and 11,493. Coexpression, deletion, and mutagenesis studies have demonstrated that this protein is encoded by ORF 1a from nucleotide 10,915 to 11,544 and is released from the 1a ...

Infectious proventriculitis syndrome is at times a serious and ongoing problem causing significant financial losses. From the obtained results in the present study, it could be concluded that proventriculitis can be transmitted by oral inoculation of homogenates produced from proventriculi of birds with proventriculitis. Experimentally inoculated broilers were seroconverted to ARV, IBV and IBDV...

The avian coronavirus (AvCoV) infectious bronchitis virus (IBV) is a major poultry pathogen. A characteristic feature of IBV is the occurrence of many different strains belonging to different serotypes, which makes a complete control of the disease by vaccinations a challenging task. Reasons for differences in the tissue tropism and pathogenicity between IBV strains, e.g. a predilection for the...

Here we introduce a culture system for the isolation, passaging and amplification of avian tracheal epithelial (ATE) cells. The ATE medium, which contains chicken embryo extract and fetal bovine serum, supports the growth of ciliated cells, goblet cells and basal cells from chicken tracheas on fibronectin- or matrigel-coated dishes. Non-epithelial cells make up less than 10% of the total popula...

Despite the existence of an active vaccination program, recently emerged strains of nephropathogenic infectious bronchitis virus (IBV) in Korea have caused significant economic losses in the poultry industry. In this study, we assessed the pathogenic and antigenic characteristics of a K-IIb type field strain of IBV that emerged in Korea since 2003, such as Kr/Q43/06. Specific pathogen free 1-we...

Montassier M.F.S., Brentano L., Montassier H.J. & Richtzenhain L.J. 2008. Genetic grouping of avian infectious bronchitis virus isolated in Brazil, based on RT-PCR/RFLP analysis of the S1 gene. Pesquisa Veterinária Brasileira 28(3):190-194. Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando de Paiva 87, São Paulo, SP 05508-000, Brazil. E-mail: leonar...

s of papers 49 and still be immunogenic. In this study, our aim is to construct a bivalent recombinant ILTV with S1 gene inserted into the TK gene region of ILTV genome, The bivalent recombinant ILTV will express S1 gene and one of the potential application is a bivalent recombinant DNA vaccine for IBV and ILTV. Firstly, TK gene and S1 gene from ILTV and IBV was isolated with PCR or RT-PCR resp...

Papain-like protease (PLpro) of coronaviruses (CoVs) carries out proteolytic maturation of non-structural proteins that play a role in replication of the virus and performs deubiquitination of host cell factors to scuttle antiviral responses. Avian infectious bronchitis virus (IBV), the causative agent of bronchitis in chicken that results in huge economic losses every year in the poultry indus...

Infectious bronchitis virus (IBV) is one of the most important threats of the poultry industry worldwide including Egypt. It is usually associated with high morbidity and mortality among the affected bird flocks. All ages and breeds of chickens are susceptible to IBV infection. Commercial chickens are highly susceptible to IBV moreover, Baladi chickens can also be infected with IBV and develop ...

S OF PAPERS 239 101 Genome sequencing and comparative analysis of avian coronavirus. M. W. Jackwood,* E. T. McKinley, J. E. Philips, and S. W. Thor, University of Georgia, Athens. Genome sequencing and comparative genomics can be used to examine past host and tissue tropism shifts to aid in identification of new variant viruses, to identify genetic differences between different pathotypes, and ...