Inhalation of hypertonic saline aerosol is a relatively noninvasive method to obtain sputum for examination of inflammatory processes in the airways. We investigated some technical factors which might influence the success of induction and sputum cell counts. In total, twenty six asthmatic and 13 healthy subjects, unable to raise sputum spontaneously, inhaled nebulized saline for three 7 min in...

INTRODUCTION In series of cases and animal models suffering hemorrhagic shock, the use of vasopressors has shown potential benefits regarding hemodynamics and tissue perfusion. Terlipressin is an analogue of vasopressin with a longer half-life that can be administered by bolus injection. We have previously observed that hypertonic albumin improves resuscitation following controlled hemorrhage i...

This article presents a methodology for acquisition and analysis of bright-field amplitude contrast image data in high-throughput screening (HTS) for the measurement of cell density, cell viability, and classification of individual cells into phenotypic classes. We present a robust image analysis pipeline, where the original data are subjected to image standardization, image enhancement, and se...

STUDY OBJECTIVES Hypertonic saline solution inhalation is suspected to produce airway inflammation. DESIGN The aim of this study was to verify this hypothesis by measuring inflammatory markers in exhaled breath condensate (EBC) collected before and after sputum induction with hypertonic and isotonic saline solution. PATIENTS AND METHODS We enrolled 10 patients with asthma, 10 patients with ...

Swenson ER: Mechanisms of improvement in pulmonary gas exchange during isovolemic hemodilution. J Appl Physiol 1999; 87:132– 41 30. Constable PD, Muir WW 3rd, Binkley PF: Hypertonic saline is a negative inotropic agent in normovolumic dogs. Am J Physiol 1994; 267:H667–77 31. Welte M, Goresch T, Frey L, Holzer K, Zwissler B, Messmer K: Hypertonic saline dextran does not increase cardiac contract...