The impact of fasting on IHL (intrahepatic lipid) content in human subjects has not been investigated previously, but results indicate that it may change rapidly in response to metabolic cues. The aim of the present study was to measure IHL content after fasting and to correlate this with circulating lipid intermediates. A total of eight healthy non-obese young males were studied before and aft...

The bacterial species Escherichia coli has proven to be a powerful tool in the molecular analysis of polyhydroxyalkanoate (PHA) biosynthesis. In addition, E. coli holds promise as a source for economical PHA production. Using this microorganism, clones have been developed in our laboratory which direct the synthesis of poly-beta-hydroxybutyrate (PHB) to levels as high as 95% of the cell dry wei...

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) were used to prepare microbeads, with diameter ranging from 50 100 µm, by an emulsion-evaporation process. The emulsification-evaporation process enables the formation of spherical polyhydroxyalcanoate beads, crystalline rates similar ones former polymer important surface roughness as compared amorpho...

A double-isotope procedure was used to measure the effects of insulin and glucagon on ketone-body production and utilization (i.e. turnover) in the starved rat. Somatostatin was infused during the experiment to suppress the pancreatic release of either hormone. The immediate action of insulin in terms of ketone-body turnover that was most evident was a decreased production of 3-hydroxybutyrate,...

I. To determine the redox state of the mitochondrial NAD system in cirrhosis of the liver and in chronic quanti tat ive undelnutrition, the substrates of the Lglutamate dehydrogenase (EC 1.4.1.2 ) and D-fl-hydroxybutyrate dehydrogenase (EC 1.1.1.3o) reactions were measured. The livers of animals fed ad l ib i tum served as controls. 2. Assuming these substrates to be in equilibrium, the NAD+/NA...

The study addresses the growth of new wild-type strain Cupriavidus necator IBP/SFU-1 and synthesis poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB) on media containing fructose three different precursors 4HB (ε-caprolactone 1,4-butanediol 1,6-hexanediol). It was found that ε-caprolactone is best precursor for P(3HB-co-4HB) copolymers. By varying concentration number doses added into ...