The hydrophobic nature of many membrane proteins, especially integral membrane proteins, brings great difficulties to their analysis. To improve the analysis of membrane proteins, an entirely solution-based combinative sample preparation (CSP) method was developed and its application to the shotgun analysis of rat liver membrane proteomes was evaluated in this study. This CSP method comprehensi...

The produced liquid water in cathode catalyst layer (CCL) has significant effect on the operation of proton exchange membrane fuel cell (PEMFC). To investigate this effect, the transport of oxygen in CCL in the presence of immiscible liquid water is studied applying a two-dimensional pore scale model. The CCL was reconstructed as an agglomerated system. To explore the wettability effects, diffe...

In this work, a mathematical model is established to describe the removal of CO2 from gaseous mixtures including CH4 and CO2 in a polypropylene hollow fiber membrane contactor in the presence of conventional absorbents such as monoethanolamine (MEA), methyldiethanolamine (MDEA), and a blend of them. Modeling was performed in axial and radial directions under the fully-wet condition for counterc...

The porosity and wettability properties of hydrogen ion treated polytetrafluoroethylene (PTFE) materials are related using contact angle, scanning electron microscopy (SEM), and ellipsometry tests. PTFE are irradiated using a low energy hydrogen ion shower (LEHIS) produced by a Gas Discharge Ion Source (GDIS). The plasma discharge current (Id) is varied at intervals of 1 mA. Results show that t...

Ionic liquids (ILs)-stabilized iron oxide (Fe(2)O(3)) nanoparticles were synthesized by the ultrasonic decomposition of iron carbonyl precursors in [EMIm][BF(4)] without any stabilizing or capping agents. The Fe(2)O(3) nanoparticles were isolated and characterized by X-ray powder diffraction, transmission electron microscopy and susceptibility measurements. The physicochemical properties of ILs...

Shallow hydrophobic insertions and crescent-shaped BAR scaffolds promote membrane curvature. Here, we investigate membrane fission by shallow hydrophobic insertions quantitatively and mechanistically. We provide evidence that membrane insertion of the ENTH domain of epsin leads to liposome vesiculation, and that epsin is required for clathrin-coated vesicle budding in cells. We also show that B...

The latest developments and applications of hollow fiber membrane contactors (HFMCs) were discussed in this recent published book. Membrane contactor provides gas–liquid or liquid–liquid contact without dispersion one phase another, which gives a higher mass transfer coefficient compared to the conventional contactors. Using microporous hydrophobic hydrophilic membrane, fluids is immobilized po...

Periplasmic binding protein-dependent transport systems are composed of a periplasmic substrate-binding protein, a set of 2 (sometimes 1) very hydrophobic integral membrane proteins, and 1 (sometimes 2) hydrophilic peripheral membrane protein that binds and hydrolyzes ATP. These systems are members of the superfamily of ABC transporters. We performed a molecular phylogenetic analysis of the seq...