background: the final objective of root canal therapy is to create a hermetic seal along the length of the root canal system. for this purpose, many methods and materials have been introduced. the purpose of this study was to compare the apical microleakage in a new obturation technique (true-tug-back) with two other obturation techniques (lateral condensation and chloroform dip technique). met...

BACKGROUND In continuation of our previously interest in the saccharification of agriculture wastes by Bacillus megatherium in solid state fermentation (SSF), we wish to report an investigation and comparative evaluation among Trichoderma sp. for the saccharification of four alkali-pretreated agricultural residues and production of hydrolytic enzymes, carboxymethyl cellulase (CMCase), filter pa...

Mycophenolate mofetil (MMF) is the ester prodrug of the immunosuppressant agent mycophenolic acid (MPA) and is rapidly activated by esterases after oral administration. However, the role of isoenzymes in MMF hydrolysis remains unclear. Although human plasma, erythrocytes, and whole blood contain MMF hydrolytic activities, the mean half-lives of MMF in vitro were 15.1, 1.58, and 3.20 h, respecti...

The importance of Glu87 and Trp89 in the lid of Humicola lanuginosa lipase for the hydrolytic activity at the water/lipid interface was investigated by site-directed mutagenesis. It was found that the effect on the hydrolytic activity upon the replacement of Trp89 with Phe, Leu, Gly or Glu was substrate dependent. The Trp89 mutants displayed an altered chain length specificity towards triglycer...

We describe the improvement of transglycosylation (TG) by chitinase D from Serratia proteamaculans (SpChiD). The SpChiD produced a smaller quantity of TG products for up to 90 min with 2 mm chitotetraose as the substrate and subsequently produced only hydrolytic products. Of the five residues targeted at the catalytic center, E159D resulted in substantial loss of both hydrolytic and TG activiti...

We recently reported that the Gibbs free energy of hydrolytic water molecules (ΔGwat) in acyl-trypsin intermediates calculated by hydration thermodynamics analysis could be a useful metric for estimating the catalytic rate constants (kcat) of mechanism-based reversible covalent inhibitors. For thorough evaluation, the proposed method was tested with an increased number of covalent ligands that ...

We studied the possibility that the antagonistic saprobe fungi Coriolopsis rigida, Trametes versicolor, Fusarium lateritium, Penicillium chrysogenum and Verticillium dahliae-2379 may control V. dahliae disease through the inhibition of the activity of hydrolytic enzymes produced by the pathogen. These saprobe fungi were able to decrease the growth in vitro of V. dahliae. The exudates produced b...

RNases are involved in critical aspects of RNA metabolism in all organisms. Two classes of RNases that digest RNA from an end (exo-RNases) are known: RNases that use water as a nucleophile to catalyze RNA degradation (hydrolytic RNases) and RNases that use inorganic phosphate (phosphorolytic RNases). It has been shown previously that the absence of the two known Escherichia coli phosphorolytic ...

A study was conducted at Universiti Putra Malaysia, Malaysia, glasshouse to determine the inoculation effect of Sb42 (locally isolated diazotroph) with different doses of nitrogen fertiliser (0, 30 and 60kg N ha) on hydrolytic enzyme production and the growth of rice variety MR219. Using 16S rRNA partial gene sequencing, Sb42 was identified as Bacillus sp. Plants were harvested at 45 days after...

Caldicellulosiruptor kronotskyensis grows on lignocellulosic biomass by the catalysis of intrinsic glycoside hydrolase, and has potential application for consolidated bioprocessing. In current study, two predicted extra- (Xyn10A) and intracellular (Xyn10B) xylanase from C. kronotskyensis were comparatively characterized. Xyn10A and Xyn10B share GH10 catalytic domain with similarity of 41%, whil...