a total of twenty seven bacterial strains were isolated from cankerous tissues of apricot, nectarine, peach, plum, sour cherry and sweet cherry trees in tehran province and identified as pseudomonas syringae pv. syringae (pss), the causal agent of the bacterial canker disease, on the basis of lopat (levan production, oxidase test, potato rot, arginine dihydrolase and tobacco hypersensitive ...

Actinidin was used to hydrolyse proteins in whey protein isolate (WPI) and milk concentrate (MPC) reduce immunoreactivity of β-lactoglobulin (β-LG) αs1-casein (αs1-CN). Samples were incubated at an enzyme substrate ratio 1:100 (5.21 units actinidin activity g−1 protein) 10 or 60 °C for up 31 5 h, respectively. Protein hydrolysis determined by the degree SDS-PAGE. Antigenicity hydrolysates β-LG ...

Considering the weak gel of tapioca starch and generally incomplete gel-advancing mechanism α-amylase modification, two commercial α-amylases (named A- N-amylase) with different reaction patterns were used to improve strength cross-linked starch. SEM showed that A-amylase exhibited surface erosion pattern, compared an extensive pattern N-amylase. XRD, SAXS, HPGPC-MALLS-RI revealed mainly attack...

Konjac oligo-glucomannan (KOG) was prepared by degradation of konjac glucomannan (KG) using β-mannanase. The hydrolysis process was monitored by the viscosity of the enzymatic hydrolysates. Factors affecting the enzymatic hydrolysis of KG were investigated, and the optimum hydrolysis conditions were as follows: time 2h; temperature 50°C; pH 6.0; and enzymatic concentration 150 U/g. Under these ...

The aim of this study was mainly on apply immobilized trypsin which covalently linked with chemically modified chitosan-coated Fe3O4 particles to producing the antihypertensive peptide by hydrolyzing peanut protein. Response Surface Methodology (RSM) was employed to optimize the hydrolysis conditions, including Enzyme/substrate ratio, temperature, pH and time. Results showed that enzyme immobil...

The best enzyme to prepare peanut peptides, papain, coupled with microwave irradiation was selected from five common proteases according to the results of the yield of peanut peptides [nitrogen solution index (NSI) in trichloroacetic acid (TCA), TCA-NSI] and the degree of hydrolysis (DH). The main factors that influenced the microwave-coupled enzymatic digestion method were optimized by respons...

The present study investigated the volatile compound, physicochemical, and antioxidant properties of beany flavor-removed soy protein isolate (SPI) hydrolyzates produced by combined high temperature pre-treatment and enzymatic hydrolysis. Without remarkable changes in amino acid composition, reductions of residual lipoxygenase activity and beany flavor-causing volatile compounds such as hexanol...

Translational GTPases (trGTPases) play key roles in facilitating protein synthesis on the ribosome. Despite the high degree of evolutionary conservation in the sequences of their GTP-binding domains, the rates of GTP hydrolysis and nucleotide exchange vary broadly between different trGTPases. EF-Tu, one of the best-characterized model G proteins, evolved an exceptionally rapid and tightly regul...

ethoxylated fatty acids or polyoxyethylene esters of fatty acids possess surface active characteristic depending on ethoxylation degree (glycol type used).they have a wide range of applications and are known by formulated trade names.fatty acids used as starting materials are obtained by the hydrolysis of fats and oils and thus the final products are fully biodegradable.the objective of this in...

in current study, protein hydrolysates were produced using microbial proteases of alcalase, protamex and flavourzyme from yellowfin tuna viscera. the results indicated that, protein hydrolysate from alcalase, had significantly higher protein content, nitrogen recovery and degree of hydrolysis than the others (p 0.05). the results of amino acids composition revealed that all hydrolysates were al...