Improvements in the synthesis, deprotection and purification of oligoribonucleotides are described. These advances allow for reduced synthesis and deprotection times, while improving product yield. Coupling times are reduced by half using 5-ethylthio-1H-tetrazole (S-ethyltetrazole) as the activator. Base and 2'-O-t-butyldimethylsilyl deprotection with methylamine (MA) and anhydrous triethylamin...

Hydrothermal process has been applied for effective production of Hydrogen from biowastes. In this study hydrothermal process for production of valuable Hydroxylapatitefrom chicken manure containing phosphorus was focused on. Conditions of 400 ̊C and 26 27 MPa with addition of 1 mmol Ca(OH)2 were determined as the optimal by using Ophospho-DL-serine as a model compound. Afterwards, the real biow...

A number of substances such as oxygen, dyes, and nitrate can act as hydrogen acceptors for xanthine oxidase (1). It has now been found that cytochrome c is also reduced by this system. Since only one other enzyme has been isolated which reduces cytochrome c (2), it was of interest to study the reaction, particularly since Ball (3) has indicated that xanthine oxidase is a flavoprotein. The activ...

Multi-walled carbon nanotubes (MWNT’s) were synthesized using chemical vapor deposition (CVD) method in a fluidized bed reactor under the flow of methane and hydrogen gases. A Cobalt-molybdenum/magnesium oxide (Co-Mo/MgO) nanocatalyst was used as the catalyst of the process. The samples were analyzed using scanning electron microscopy (SEM) and X-ray diffraction (XRD) analyses. The effects of d...

The reduced form of thioredoxin, a low molecular weight hydrogen transport protein, was isolated from calf liver. The purification involved heat treatment and chromatography on DEAE-cellulose, Sephadex G-SO, and CM-cellulose, and it resulted in an apparently pure protein after a 4,000-fold purification with a final yield of 30%. The thioredoxin preparation obtained was homogeneous as judged by ...

The pressure swing adsorption (PSA) system is widely applied to separate and purify hydrogen from gaseous mixtures. extended Langmuir equation fitted the Langmuir-Freundlich isotherm has been used predict isothermal of methane on zeolite 5A adsorbent bed. A six-step two-bed PSA model for purification developed validated by comparing its simulation results with other works. effects pressure, P/F...

Graphene films were fabricated over synthesized MCM-41 nanocatalyst by chemical vapordeposition method, and the reaction was carried in atmospheric pressure at 750˚C. Acetylenegas used as a carbon precursor and the synthesis reaction took place in hydrogen atmosphere.Mesoporous MCM-41 was synthesized at room temperature, using wet chemical method. Thesynthesized metal free catalyst was characte...

Currently, much of our biodegradable wastes such as kitchen wastes, agricultural wastes & animal wastes are used to produce Biogas, a powerful greenhouse gas. Anaerobic digestion (AD) is a treatment that composts these wastes in the absence of oxygen, producing a biogas that can be used to generate Heat & Power. Producing renewable energy from our biodegradable wastes helps to tackle the energy...

Single-walled carbon nanotubes (SWNTs) have unique properties and are projected to have a major impact in nanoscale electronics, materials science, and nanomedicine. Yet, these potential applications are hindered by the need for sample purification to separate SWNTs from each other and from metallic catalyst and amorphous carbon present in as-synthesized samples. Common purification strategies ...