Interspecific hybrids of Pleurotus florida PF-5 (MTCC 1801) and Pleurotus sajor-caju PSC3 (ATCC 62887) were developed using electrofusion. A concentration of 2mM vanillin and 1000ppm carbendazim were selected as screening markers for the hybrids of the two Pleurotus spp. The electrofusion using an exponential decay was best with 1000 V (2 pulses with an interval of 5 sec). With square wave disc...

Leaves of 13 interspecific and intergeneric citrus somatic allotetraploid hybrids were analysed and compared with those of their respective parents (lime, lemons, citron, orange, kumquat, poncirus and mandarin) with regards to their sesquiterpenoid contents (μg/g dry weight). The hybrids do not fully retain their parental traits. Sesquiterpenoid production is much reduced (by ~65–90%) in hybrid...

The study of grain hybrids with faster or more extensive rates of ruminal starch fermentation has been a key research area. Because grain sorghum starch is generally regarded as less accessible to enzymatic degradation than starch in other grains, it has received the greatest research emphasis. However, all grains have been evaluated to some extent. Grain sorghum hybrids appear to be more varia...

1. The main aim of the present review is to raise awareness of the molecular complexity of single skeletal muscle fibres from "normal" and "transforming" muscles, in recognition of the many types of hybrids that have been observed in vertebrate skeletal muscle. The data used to illustrate various points made in the review were taken from studies on mammalian (mostly rat) and amphibian muscles. ...

RNA-DNA hybrids are a major internal cause of DNA damage within cells, and their degradation by RNase H enzymes is important for maintaining genomic stability. Here, we identified an unexpected role for RNA-DNA hybrids and RNase H enzymes in DNA repair. Using a site-specific DNA double-strand break (DSB) system in Schizosaccharomyces pombe, we showed that RNA-DNA hybrids form as part of the hom...

Interspecific hybridization in Tecoma Juss. was conducted to develop novel forms for the nursery industry. We report fertile hybrids from the cross T. garrocha Hieron. (pistillate parent) × T. stans (L.) Juss. ex Kunth. Leaf morphology of the F(1) hybrids of T. garrocha × T. stans was intermediate between the parents. GISH also confirmed hybridity. The F(1) hybrids were successfully backcross...

exploitation of the full potential of any hybrid requires the possessing of genetically high-purity seeds. commercial soybean hybrids have been developed using a cytoplasmic male sterility (cms) system. in order to avoid reduction in yield caused by using low-purity seeds, development of a simple, rapid, and accurate method for hybrid purity assessment is of great essence and significance. ther...

Hybrids between Rhipicephalus appendiculatus and R. zambeziensis were reared and glucose-phosphate-isomerase isoenzymes were resolved by agarose electrophoresis. By phenotyping hybrids in F1 and F2 generations autosomal transmission of 2 GPI genes was demonstrated. Identification of a hybrid phenotype provides a method for identifying hybrids in field collections.

The role of pollinators in plant speciation and maintenance of species boundaries is dubious because most plant species are visited by several types of pollinators, and most pollinator species visit several species of plants. We investigated pollinator preferences and their efficacy as ethological isolation mechanisms between two interfertile species, Nicotiana alata and N. forgetiana and their...

The intersubspecific hybrids of autotetraploid rice has many features that increase rice yield, but lower seed set is a major hindrance in its utilization. Pollen sterility is one of the most important factors which cause intersubspecific hybrid sterility. The hybrids with greater variation in seed set were used to study how the F(1) pollen sterile loci (S-a, S-b, and S-c) interact with each ot...