BACKGROUND Oral cavity is a doorway for a variety of products containing titanium dioxide (TiO2 ) nanoparticles (NPs) (nano-TiO2 ) such as food additives, oral healthcare products and dental materials. Their potential to penetrate and affect normal human oral mucosa is not yet determined. OBJECTIVES To evaluate the ability of nano-TiO2 to penetrate the in vitro reconstructed normal human bucc...

Carcinoma associated fibroblasts (CAFs) have recently been implicated in neoplastic progression, tumor growth, angiogenesis, and metastasis. This special fibroblast subpopulation plays a major role in initiation of tumor progression of head and neck squamous cell carcinoma. We established a co-culture model of SCC-25 lingual squamous cell carcinoma cells and periodontal ligament fibroblasts. Ge...

background: human papillomavirus (hpv) and p53 alterations are speculated to play a role in carcinogenesis. this study was carried out to find out the association of hpv and p53 with precancerous lesions of the oral cavity such as leukoplakia: the objective of this study was to find the association among human papilloma virus (hpv) 16 infections and p53 polymorphism in tobacco using the oral le...

BACKGROUND AND OBJECTIVE The cell-surface lipooligosaccharide (LOS) of Treponema denticola possesses several biological properties. The aim of this study was to investigate the binding properties of T. denticola LOS to extracellular matrix (ECM) proteins, mucosal cells, and oral bacteria. DESIGN LOS was isolated from T. denticola and labeled with tritium. Tritium-labeled LOS was placed in ECM...

Abstract The oral cavity is a unique stricture; it combines mucosal and bone tissue microenvironments with their respective immunological responses. In fact, inflammation leads to destruction in the prevalent human disease, periodontitis. periodontitis, immune cells, particular Th17 neutrophil mediated responses have been linked pathogenesis, however not yet understood how stromal microenvironm...

A method based on manipulation of the human cytomegalovirus genome in a bacterial artificial chromosome was developed to determine the role played by 6 UL97 mutations of unknown significance. These mutations were found in blood samples from solid-organ transplant recipients in a trial comparing valganciclovir and oral ganciclovir prophylaxis. Recombinant viruses containing UL97 mutations P405L,...

Nickel-chromium (Ni-Cr) alloys used in fixed prosthodontics have been associated with type IV Ni-induced hypersensitivity. We hypothesised that the full-thickness human-derived oral mucosa model employed for biocompatibility testing of base-metal dental alloys would provide insights into the mechanisms of Ni-induced toxicity. Primary oral keratinocytes and gingival fibroblasts were seeded onto ...

Background  Recent investigations show that both proliferation and secretion of macromolecules by cells can be regulated by low level laser therapy (LLLT). The aim of this study was to determine whether LLLT could induce a bio-stimulatory effects on human gingival fibroblasts (HGF3-PI 53). Therefore, the effect of laser irradiation on human gingival cell proliferation and collagen type I gene ...

Bone morphogenetic protein-2 (BMP-2)-containing bone grafts are useful regenerative materials for oral and maxillofacial surgery; however, several in vitro and in vivo studies previously reported cancer progression-related adverse effects caused by BMP-2. In this study, by quantifying the rhBMP-2 content released from bone grafts, the rhBMP-2 concentration that did not show cytotoxicity in each...