The objective of this study was to investigate the relationship between heat shock protein 70 (HSP70) overexpression and intestinal structure and digestive function in heat-stressed broilers. In total, 240 male broilers were injected intraperitoneally with l-(1)-glutamine (0.75 mg/kg of BW) or quercetin (5 mg/kg of BW). Twenty-four hours later, they were heat-stressed for 0, 2, 3, 5, and 10 h, ...

A feeding trial has been conducted to evaluate the effects of dietary nucleotide (NT) on growth performance and stress resistance of fry kutum (Rutilus kutum Kamenskii, 1901). A basal diet supplemented with 0 (control), 0.3, 0.5 and 0.7 g NT kg-1 have been formulated for four experimental diets. After 8 weeks of feeding trial, growth parameters were evaluated and then fish has exposed to osmoti...

BACKGROUND The issue of heat shock protein (HSP) 70 and anti-HSP70 antibodies in chronic rhinosinusitis (CRS) has never been explored. OBJECTIVE To determine the nasal secretion (NS) levels of HSP70 and anti-HSP70 antibodies in patients with CRS with nasal polyps (CRSwNP) and patients with CRS without nasal polyps (CRSsNP), and to evaluate their associations with CRS clinical severity and cor...

BACKGROUND Heat-shock proteins (HSPs) protect cells and tissues against different types of damage. This study investigated the relationship between the genetic variation in constitutive and inducible HSP70 genes and the risk for coal workers' pneumoconiosis (CWP) among miners in southwestern China. PATIENTS AND METHODS A job exposure matrix (JEM) was used to estimate the dust exposure level o...

The functional relationship between thermotolerance and heat shock protein (HSP) expression is well described. However, the intraspecific variation in HSP70 expression and heat resistance is not fully understood. Some studies have shown a correlation between thermotolerance and HSP70 expression levels at the intraspecific level, whereas others have not, and results depend on species, developmen...

BACKGROUND AND PURPOSE This study investigated the effects of intravenous recombinant Fv-Hsp70 protein on infarction volume and behavior after experimental ischemic stroke. METHODS Focal cerebral ischemia was produced by occluding the middle cerebral artery using the intraluminal suture technique. Rats subjected to 2 hours of focal ischemia were allowed to survive 24 hours. At 2(1/4) hours an...

Molecular chaperones of the Hsp70 family form an important hub in the cellular protein folding networks in bacteria and eukaryotes, connecting translation with the downstream machineries of protein folding and degradation. The Hsp70 folding cycle is driven by two types of cochaperones: J-domain proteins stimulate ATP hydrolysis by Hsp70, while nucleotide exchange factors (NEFs) promote replacem...

Resistance to stress-induced apoptosis was examined in cells in which the expression of hsp70 was either constitutively elevated or inducible by a tetracycline-regulated transactivator. Heat-induced apoptosis was blocked in hsp70-expressing cells, and this was associated with reduced cleavage of the common death substrate protein poly(ADP-ribose) polymerase (PARP). Heat-induced cell death was c...

In this study, we investigated the role of the inducible form of heat shock protein 70 (hsp70) in the presentation of the major putative autoantigen in multiple sclerosis, myelin basic protein (MBP), in the context of appropriate MHC class II. By coimmunoprecipitation, we found that MBP is associated with hsp70 in APC in an ATP/ADP-dependent manner. Additionally, using confocal microscopy, hsp7...

The heat shock proteins (HSPs) are ubiquitous molecules induced in cells exposed to various stress conditions, including carcinogenesis. The HSP70 and HSP27 among HSPs are of special relevance in human cancer inhibiting apoptosis. The aim of this study is to investigate the expressions of HSP70 and HSP27 in hepatocellular carcinoma (HCC) in association to tumor cell proliferation and apoptosis....